Synthesis of CuO Nanoparticles by using Leaf Extracts of Melia azedarach and Morus nigra and their Antibacterial Activity
Synthesis of CuO Nanoparticles by using Leaf Extracts of Melia azedarach and Morus nigra and their Antibacterial Activity
Tahira Moeen Khan* and Amat ul Mateen
ABSTRACT
Nanoparticles are gaining popularity due to their increasing biomedical applications as anti-bacterial, anti-fungal and anti-cancer agents. In this work leaf extracts of Melia azedarach (commonly known as dharaik) a species of family Meliaceae and Morus nigra (commonly known as Shahtoot) family Moraceae were used for bio reduction of copper ions to CuO nanoparticles in place of harmful and expensive chemical/physical methods. A cheaper, simple to perform method giving high yield without by products is employed here. The leaf extract act both as reducing and capping agent. The atomic absorption shows a linear curve between concentration and absorbance. The uv-vis spectrum revealed the shift of absorption towards longer wavelength with the increasing concentration of plant extract. XRD indicates CuO Nps particle size is in between 14-20 nm. CuO nanoparticle show very significant antibacterial activity against four bacterial strains three gram negative (Escherichia coli, Proteus mirabilis, Salmonella) and one gram positive (Clostridium tetani). The minimum concentration of leaf extracts needed against these bacterial strains were also calculated. The present study indicated that Melia azedarach and Morus nigra leaf extracts can be used successfully for nanoparticles synthesis and CuO as significantly active antibacterial material. Antibacterial activity of these nanoparticles was observed by agar well diffusion method.
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December 2018
Vol. 4, Iss. 2, Pages 83-135
