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Synergistic Effect of Entomopathogenic Fungi and Bacteria against Pulse Beetle, Callosobruchus chinensis

Synergistic Effect of Entomopathogenic Fungi and Bacteria against Pulse Beetle, Callosobruchus chinensis

Mohsan Iqbal1, Farid Asif Shaheen1, Rashid Mahmood2,*, Muhammad Khalid Rafique2, Imran Bodlah1, Farah Naz3 and Muhammad Usman Raja3

1Department of Entomology, PMAS-Arid Agriculture University, Rawalpindi
2Honeybee Research Institute, National Agricultural Research Centre, Islamabad
3Department of Plant Pathology, PMAS-Arid Agriculture University, Rawalpindi

*      Corresponding author: rashid_ento1@yahoo.com

 

ABSTRACT

Callosobruchus chinensis is one of the most destructive insect pests of Cicer arietinum (Chickpea) in storages and renders grains unfit for human consumption. In the present study, synergistic effects of entomopathogenic fungi (EPF), Beauveria bassiana and Metarhizium anisopliae and entomopathogenic bacteria (EPB), Photorhabdus temperata and Xenorhabdus nematophila were studied as bio-control agents to manage this pest. In addition, percent conidial germination and % mortality of C. chinensis were also evaluated when B. bassiana was used with DEBBM. The minimum number of eggs (0.66 grain-1), number of holes (1 grain-1), number of F1 new emerged (5.6 jar-1), days to 100 percent mortality F1 (3), weight loss (4%), damage (5%) whereas the maximum inhibition rate of 85% and percent mortality of C. chinensis were observed in synergistic concentration of EPB as compared toEPF. Synergistic concentration (1×108) of bacteria gave the best results against C. chinensis as compared to that of entomopathogenic fungi. The maximum percent conidial germination (90%) of B. bassiana and percent mortality (50.40±2.20a), (79.70±0.28a) and (90.76±0.56a) of C. chinensis were observed when DEBBM was synergized with B. bassiana after seven, fourteen and twenty one days, respectively.
 

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Pakistan Journal of Zoology

October

Vol. 51, Iss. 5, Pages 1599-1997

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