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Studies on the Physical Parameters for Cephalosporin Biosynthesis from Acremonium chrysogenum by Submerged Fermentation

Studies on the Physical Parameters for Cephalosporin Biosynthesis from Acremonium chrysogenum by Submerged Fermentation

Umar Farooq Gohar1, Hamid Mukhtar1,*, Ali Nawaz1, Ikram-ul-Haq1 and Asad Mehmmood2

1Institute of Industrial Biotechnology, GC University, Lahore-54000, Pakistan
2 School of Chemistry, University of New South Wales, Australia

*      Corresponding author: hamidwaseer@yahoo.com

 

ABSTRACT

The present study was performed to optimize physical parameters for the maximum production of antibiotic (cephalosporin) by isolated fungal strain Acremonium chrysogenum (IIB-10). The strain IIB-10 was cultivated on medium containing (%, w/v): Baker’s flour, 1.5; corn meal, 2; Methyl oleate, 1.6; calcium carbonate, 0.3 and ammonium sulphate, 0.1. The cultural conditions such as incubation temperature (30°C), pH (6.5) and inoculum size and age (2%, w/v, 48 h) were optimized. The maximum cephalosporin production was achieved after 144 h of incubation. The dry mycelial mass was found to be 39.46 mg/ml. The antimicrobial activity was assessed by disc diffusion method. Cephalosporin production was estimated by using spectrophotometric technique.
 

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Pakistan Journal of Zoology

April

Vol. 51, Iss. 2, Pages 399-799

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