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Storage of Bacteriophages at 4°C Leads to no Loss in Their Titer after One Year

Storage of Bacteriophages at 4°C Leads to no Loss in Their Titer after One Year

Iqbal Ahmad Alvi, Muhammad Asif, Rabia Tabassum, Zaigham Abbas and Shafiq ur Rehman*

Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore 54590

*     Corresponding author: shafiq.mmg@pu.edu.pk

 

ABSTRACT

The storage of bacteriophages is tricky as no ideal common practice is available and the storage conditions may differ from phage to phage. There is disparity among the scientific community regarding bacteriophage storage conditions and various conditions (4 oC, -20 oC, -80 oC and liquid nitrogen freezing at -196°C) are used by different laboratories. In this study, the storage stability of five bacteriophages, JHP, RLP, RSP, SaPL, and IttPL, at distinct temperatures for complete one year is described. Our results indicated that storage of bacteriophages at 4oC was most appropriate, followed by storage at -20oC and -80oC as no drop in viral titer was witnesses in bacteriophage samples stored at 4oC for up to one year. The results of this research indicated, that a standard refrigeration is suitable and economical means of long term storage with no fall of phage activity.
 

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Pakistan Journal of Zoology

December

Vol. 50, Iss. 6, Pages 1999-2398

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