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Spotting rs112445441 in Non-Hodgkin Lymphoma: Another Clue for the Context-Dependent Crosstalk between RAS-MAPK and PI3K Mediated Pathways

Spotting rs112445441 in Non-Hodgkin Lymphoma: Another Clue for the Context-Dependent Crosstalk between RAS-MAPK and PI3K Mediated Pathways

Bibi Nazia Murtaza1, Mazhar Saeed Chaudry2, Shamaila Inayat Nadeem1, Muhammad Shahid Nadeem3 and Abdul Rauf Shakoori4,*

 

1Department of Zoology, Kinnaird College for Women University, Lahore 54000 

 

2Services Institute of Medical Sciences, Lahore 54000 
3Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia
4Department of Biochemistry, Faculty of Life Sciences, University of Central Punjab, Johar Town, Lahore

*      Corresponding author: arshaksbs@yahoo.com; 

dr.arshakoori@ucp.edu.pk

 

Fig. 1.

Scheme of Hydrogen bonding between β-phosphate of GDP and backbone of P-loop (residues 13–17) in WT (left) and G13D (right) (Figure adapted from Lu et al., 2015). RAS family is direct or indirect regulator of complex signalling mechanism like, RAS-ERK-MAPK, PI13K, ral guanine nucleotide dissociation stimulator (RALGDS) and protein kinase C (PKC) etc. (Khan et al., 2018), hence involved in plethora of molecular activities associated with normal cellular functions.

Fig. 2.

A, Sequence alignment by BLAST; B, 12 % Acrylamide: bisacrylamide electrophoresis photograph showing DNA fragment containing codon 13 after restriction. Sample lanes 1-7 and 9, wild type; lane 8, NHL (heterozygous mutant); and lane M is 50 bp DNA marker (Fermentas); C, Sequencing shwoing GGC (glycine) to GAC (aspartic acid).

Fig. 3.

Context-dependent crosstalk between RAS-MAPK and PI3K mediated pathways. (Figure adapted).

Fig. 4.

The structures of (A) WT, (B) G12D and, (C) G13D KRAS proteins are drawn in cartoon putty representations at the P-loop, switch I and II regions; blue represents the lowest and red the highest B-factor value. In addition, the size of the tube reflects the value of the B-factor, in that the larger the B-factor, the thicker the tube. The structures in the other regions are coloured in white and displayed in cartoon tube representation, where the size of the tube is independent of the B-factors (Figure adapted from Chen et al., 2013).

Pakistan Journal of Zoology

February

Vol. 52, Iss. 1, Pages 1-424

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