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Species Identification of Gram Wilt Complex through ITS Region by PCR-RFLP Analysis

Muhammad Jawad1*, Shahid Riaz Malik1, Rana Muhammad Atif2, Haris Ahmed2 and Muhammad Shahzad Afzal3

1Crop Science Institute, Pakistan; 2University of Agriculture, Faisalabad, Pakistan; 3Sugarcane Research Institute, AARI, Faisalabad, Pakistan.

*Correspondence | Muhammad Jawad, Crop Science Institute, Pakistan; Email:

Figure 1:

UPGMA dendrogram of collected isolates based on Universal Rice Primers.

Figure 2:

Agarose gel showing (A) amplification of ITS regions (ITS-1 and ITS-4) and restriction pattern of PCR-amplification product digested with HhaeI (B); BsuRI (C); HinfI (D) and MboI (E).

Pakistan Journal of Agricultural Research


Vol. 34, Iss. 2, Pages 254-493


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