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Single Nucleotide Polymorphism in the Promoter Region of the IGF-1 Gene is Associated with Milk Production in Holstein and Jersey Cattle – Is the Aspect of Present Research Still Relevant in the Era of Genomic Selection?

Single Nucleotide Polymorphism in the Promoter Region of the IGF-1 Gene is Associated with Milk Production in Holstein and Jersey Cattle – Is the Aspect of Present Research Still Relevant in the Era of Genomic Selection?

Ewa Czerniawska-Piątkowska1, Iwona Szatkowska1, Daniel Zaborski2*, Wilhelm Grzesiak2, Sara Tabor-Osińska1, Małgorzata Wasielewska1Witold S. Proskura1, Wojciech Kruszyński3 and Edward Pawlina3

1Laboratory of Molecular Cytogenetics, Department of Ruminants Science, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Szczecin, Klemensa Janickiego 29, 71-270 Szczecin, Poland 
2Laboratory of Biostatistics, Department of Ruminants Science, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Szczecin, Klemensa Janickiego 29, 71-270 Szczecin, Poland 
3Department of Genetics, Faculty of Biology and Animal Breeding, Wrocław University of Environmental and Life Sciences, Kożuchowska 7, 51-631 Wrocław, Poland

*      Corresponding author: daniel.zaborski@zut.edu.pl

ABSTRACT

The aim of the present study was to verify the potential effect of the C>T substitution at position -512 in the regulatory region of the IGF-1 gene on the level of milk performance traits in different cattle breeds. The study involved 227 Jersey, 147 Polish Holstein-Friesian black-and-white (HO) and 181 Polish Holstein-Friesian red-and-white (RW) cows. PCR-RFLP was used for genotyping. A bioinformatic analysis of the P1 regulatory sequence was also carried out. Three genotypes (TT, CT and CC) were identified. The CT genotype was the most frequent (0.40, 0.50 and 0.52 in Jerseys, HO and RW, respectively). The frequencies of the CC genotype were 0.22, 0.28 and 0.31 in the RW, Jersey and HO breeds, respectively, whereas those of the TT genotype were 0.19, 0.26, and 0.32 for HO, RW, and Jerseys, respectively. For Jerseys, significant differences in milk yield, fat and protein percentage were found in the second lactation. In HO, significant differences in milk fat content and yield were observed in the third lactation. No significant differences were found for RW. The bioinformatic analysis allowed us to infer that transcription factors other than the ZFP217 protein bind to the sites located outside the C>T substitution. Further studies are required to elucidate the molecular basis of the relationships observed in the present research.
 

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Pakistan Journal of Zoology

October

Vol. 53, Iss. 5, Pages 1603-2000

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