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Serological Prevalence of Hepatitis B Virus in Peshawar, Khyber Pakhtunkhwa, Pakistan

Serological Prevalence of Hepatitis B Virus in Peshawar, Khyber Pakhtunkhwa, Pakistan

Tanweer Kumar1, Sahib Zada2,3,Muhammad Irfan4,*, Huma Batool5 and Wasim Sajjad2,6

1PARC Institute for Advanced Studies in Agriculture, National Agricultural Research Centre, Park Road, Islamabad, Pakistan
2Department of Microbiology, Quaid-i-Azam University, Islamabad 45320, Pakistan
3Department of Allied Health Sciences, Iqra National University, Peshawar, Pakistan
4Department of Microbiology and Cell Science Genetics, Institute and Institute of Food and Agricultural Science, University of Florida, Gainesville, FL, USA
5Deprtement of Botany, Sardar Bahadur Khan Women’s University, Quetta, Balochistan, Pakistan
6State Key Laboratory of Cryosphere Science, Northwest Institute of Eco-Environment and Resources, University of Chinese Academy of Sciences, China

*      Corresponding author:



The present study aims to investigate an up-to-date prevalence of Hepatitis B Virus (HBV) infection among a mix population of Peshawar, Khyber Pakhtunkhwa, Pakistan. This study highlighted the relationship between HBV, demographic, clinical parameters and the risk factors in patients with HBV. A total of 4,758 blood samples from the mixed population were collected including 2382 male 2376 females. All the samples were subjected to hepatitis B surface antigen Immuno-chromatographic test (ICT), HBsAg enzyme-linked immunosorbent assay and by Reverse transcription polymerase chain reaction for the existence of antibodies against HBV. It was observed that 307 (12.8%) out of the 2382 individuals harbored antibodies in their blood against HBV. Among the different age groups, the highest number of incidences of HBV antibodies was found in the 16-25 age groups (7.01%). ICT positive samples were further screened by nested polymerase chain reaction (PCR) to determine the existence of active HBV-DNA. It was investigated that 5.7% (2382) of the total population (4758) tested was positive, among which the female 15.4% (367) possessed antibodies in their blood against HBV. Our results showed a higher male percentage than that of the female. The total HBV prevalence was recorded at 4.5% in all populations comprising both male and female. It was concluded that the highest prevalence of HBV was found in male and female in the age group 16-25, and then followed by the age group that lies between 26-55.

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Pakistan Journal of Zoology


Vol. 55, Iss. 1, Pages 1-500


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