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Separation of Enzymes from their Aqueous System by using Novel Concept of Unidirectional Freezing

Separation of Enzymes from their Aqueous System by using Novel Concept of Unidirectional Freezing

Muhammad Waheed Mushtaq1,*, Muhammad Waqar Younas2, Jamil Anwar2, Syed Mustansar Abbas3 and Shahid Bashir3

1Institute of Chemistry, University of the Punjab, Lahore - 54590
2Department of Chemistry, Government College of Science, Wahdat Road, Lahore
3Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia

*      Corresponding author: waheedjaami@gmail.com

 

ABSTRACT

In our present work, the separation and extraction of three different enzymes (alpha-amylase, cellulase and pectinase) from aqueous system has been studied by applying novel concept of unidirectional freezing. To separate the enzymes from their aqueous system, directional freezing was applied horizontally through synthetic enzyme contaminated water samples, called radial freezing. After complete freezing, the samples were collected from various sections of the frozen mass and concentration of enzymes was determined. It was found that more than ~85% mass of enzymes, migrated along with freezing front and concentrated in the centre in case of radial freezing. To examine the effect of unidirectional freezing over extracted enzymes, the working activity of separated enzymes was checked by performing desizing test for alpha-amylase and biopolishing test for cellulase. The noteworthy results of desizing and biopolishing test has promoted the significance of unidirectional freezing technique (UFT).
 

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Pakistan Journal of Zoology

October

Vol. 51, Iss. 5, Pages 1599-1997

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