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Separation of Bacterial Contaminations in Aqueous Systems by Directional Freezing

Separation of Bacterial Contaminations in Aqueous Systems by Directional Freezing

Muhammad Waheed Mushtaq1,*, Jamil Anwar2, Omara Naeem3,Farah Kanwal1 and Waheed-uz-Zaman1

1Institute of Chemistry, University of the Punjab, Lahore 54590
2School of Physical Sciences, University of the Punjab, Lahore 54590
3Department of Biology, Lahore Garrison University, Lahore

*     Corresponding author: waheedjaami@gmail.com

 

ABSTRACT

Directional freezing was applied horizontally as well as vertically for separation of bacterial contamination in aqueous systems. After complete freezing, the samples were collected from various sections of the frozen mass and bacterial colonies were counted after proper Gram staining. It was found that more than 90% bacteria were separated along with freezing front and were concentrated in the centre in the case of radial freezing and at the bottom in case of vertical freezing.
 

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Pakistan Journal of Zoology

October

Vol. 50, Iss. 5, Pages 1601-1998

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