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Role of Cumulus Cells and Follicular Fluid on Oocyte Maturation and Developmental Competence of Embryos: Intact and Reconstructed Oocytes

Role of Cumulus Cells and Follicular Fluid on Oocyte Maturation and Developmental Competence of Embryos: Intact and Reconstructed Oocytes

Rashid Al Zeidi1, Haitham Al Masruri2, Aiman Al Mufarji3*, Abd El-Nasser Ahmed Mohammed3 

1Department of Clinical Studies, College of Veterinary Medicine, King Faisal University, P.O. Box 400, Al-Hassa, Kingdom of Saudi Arabia; 2Department of Public Health and Animal Care, College of  Veterinary Medicine, King Faisal University, P.O. Box 400, Al-Hassa, Kingdom of Saudi Arabia; 3Department of Animal and Fish Production, College of Agriculture and Food Sciences, King Faisal University, P.O. Box 400, Al-Hassa, Kingdom of Saudi Arabia.

*Correspondence | Aiman Al Mufarji, Department of Animal and Fish Production, College of Agriculture and Food Sciences, King Faisal University, P.O. Box 400, Al-Hassa, Kingdom of Saudi Arabia; Email: 220034536@student.kfu.edu.sa  

Figure 1

Differentiation, proliferation and development of ovarian structures during fetal life 

Figure 2

Growth of preantral gonadotropin-independent follicles and antral gonadotropin-dependent follicles after birth.
FSH, Follicle stimulating hormone
LH; Luteinizing hormone
GDF9; Growth differentiation factor 9
bFGF; basic fibroblast growth factor
BMP15; bone morphogenetic protein 15 

Figure 3

Ovarian follicle structures of mice; secondary follicles (A), graaffian follicles (B) and cumulus-enclosed germinal vesicle oocytes (Mohammed AA) 

Figure 4

Mice denuded germinal vesicle oocytes (A) and cumulus-enclosed germinal vesicle oocytes (B) 

Figure 5

Complete and selective enucleation of denuded and cumulus-enclosed germinal vesicle oocytes; complete enucleation with removal the whole nucleus (A,B) and selective enucleation leaving the nuclear sap and nucleolus in the cytoplast (C,D).  

Advances in Animal and Veterinary Sciences

May

Vol. 12, Iss. 5, pp. 802-993

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