The study was conducted to evaluate the effectiveness of neem seed extract (NSE) for the management of Bemesia tabaci Gennadius, (Homoptera: Aleyrodidae) and Amrasca biguttula Ishida, (Homopter: Cicadellidae) infesting Bt and non-Bt cotton cultivars. Foliar application of neem seed, Azadirachta indica extract was applied upon reaching economic threshold levels of B. tabaci and A. biguttula. The insect pest population was recorded 24 hours before and 24h, 72h and 168h after spray. Maximum reduction of 60.20% of B. tabaci on Bt cotton was recorded at 6% NSE while at 2% concentration of NSE after 148 hrs, 39. 16% reduction was observed. While maximum reduction on non-Bt cotton at 6% recorded as 66.60% and minimum at 2% concentration recorded as 48.72% of neem seed extract against B. tabaci. In case of A. biguttula, maximum reduction in population was observed at 6% concentration of NSE (64.94%) and minimum at 2% concentration (44.50%) on Bt cotton. While maximum reduction of A. biguttula was observed at 6% concentration (69.05%) and minimum at 2% concentration (48.48%) on non-Bt cotton.