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Regulation of Collagen Metabolism in Keratinocyte-Fibroblast Organotypic Co-culture by Pressure

Regulation of Collagen Metabolism in Keratinocyte-Fibroblast Organotypic Co-culture by Pressure

Yang Liu1,2,3, Baimei Liu1,2,3, Chenchen Li1,2,3 and Meiwen An1,2,3,*

1Institute of Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, China
2Shanxi Key Laboratory of Material Strength and Structural Impact, College of Mechanics, Taiyuan University of Technology, Taiyuan 030024, China
3National Demonstration Center for Experimental Mechanics Education, Taiyuan University of Technology, Taiyuan 030024, China

*      Corresponding author: zi_su_tai_yuan@163.com

 

Fig. 1.

A, SEM morphology of CG three-dimensional scaffolds; B, keratinocytes; C, Fusion of fibroblasts; D keratinocytes morphology on CG scaffolds, HE stains of group B (KM in KM&FM without pressure); E, fibroblasts morphology on CG scaffolds, HE stains of group F (FM in KM&FM without pressure).

Fig. 2.

A, the comparation expression of type I collagen mRNA in KM; B, type III collagen mRNA expression in KM; C, type I collagen mRNA expression in FM; D, type III collagen mRNA expression in FM; E, production of type I collagen; F, production of type III collagen: a to d shows significant difference of protein expression of KM (*P<0.05), and e and f shows significant difference of protein expression of FM (*P<0.05). n=3.

Fig. 3.

A, the comparation expression of IL-1α mRNA in KM; B, the comparation expression of IL-1α mRNA in FM; C, the production of IL-1α; D, the comparation expression of MMP-3 mRNA in KM; E, the comparation expression of MMP-3 mRNA in FM; F, the production of MMP-3: a to d shows significant difference of protein concentration of KM (*P<0.05), and e and f shows significant difference of protein concentration of FM (*P<0.05). n=3.

Pakistan Journal of Zoology

December

Vol. 50, Iss. 6, Pages 1999-2398

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