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RNA-Seq Reveals Differentially Expressed Genes of Pig Vaccinated with Modified Live Attenuated Porcine Epidemic Diarrhea

RNA-Seq Reveals Differentially Expressed Genes of Pig Vaccinated with Modified Live Attenuated Porcine Epidemic Diarrhea

Sung Kwon Park1a, Jin Young Jeong2a, Eun Seok Cho3, Yong Dae Jeong3 and Chang Seok Park4*

1Department of Food Science and Technology, Sejong University, 209, Neungdong-ro, Gwangjin-gu, Seoul, Republic of Korea
2Animal Nutrition & Physiology Team, National Institute of Animal Science, RDA, 1500, Kongjwipatjwi-ro, Wanju-gun, Jeollabuk-do, 55365, Republic of Korea
3Swine Science Division, Department of Animal Resources Development, National Institute of Animal Science, RDA, Cheonan, Republic of Korea
4Hanwoo Research Institute, National Institute of Animal Science, RDA, Pyeongchang 232-950, Republic of Korea

aSung Kwon Parkand Jin Young Jeong contributed equally to this work.

*        Corresponding author: phj3578@korea.kr 

ABSTRACT

Porcine epidemic diarrhea (PED) was triggered by pathogenic coronavirus causing watery diarrhea and high mortality in piglets. This post-viral syndrome results in severe economic losses in the pig industry. However, limited information on the porcine epidemic diarrhea virus-infected pigs is available. Thus, the objective of this study was to identify transcriptomes with RNA sequencing (RNA-seq) in peripheral blood mononucleated cell (PBMC) of piglets, Large White x Landrace crossbreds, infected with live strain PED virus (PEDV). Several gene ontology (GO) and differentially expressed gene (DEG) are also identified by RNA-seq. The analyzed transcriptome provides the 1805 DEGs and gene networks involving cellular processes. Between vaccinated and unvaccinated groups, there were 13 and 17 up- and down-regulated DEGs (2-fold; FDR < 0.05; P < 0.001), respectively, in PBMC of PEDV. Most of significant DEGs were involved in multiple cellular processes of cell growth and immune system. Thus, results from our current study indicate that DEG analysis of RNA-seq data help us better understand the precise mechanisms of PEDV in pig PBMC. 
 

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Pakistan Journal of Zoology

December

Vol. 51, Iss. 6, Pages 1999-2399

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