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Proteomic and Immunological Identification of Antigens from Ticks with Artificial Antiserum

Proteomic and Immunological Identification of Antigens from Ticks with Artificial Antiserum

Ke Zhang1, Shuai Liu2, Qiu Chen1, Yu Wang1, Li Liu1, Bingjie Li1, Kai Ma1, Xiaoya Wei1, Aijun Li3 and Junyan Li2*

1Medical College, Pingdingshan University, Pingdingshan 467000, China
2National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100000, China
3Pingdingshan Center for Disease Control and Prevention, Pingdingshan 467000, China
 
Ke Zhang and Shuai Liu contributed equally to this work.

* Corresponding author: lijunyan688@126.com

ABSTRACT

We aimed to screen novel tick antigens using two-dimensional gel electrophoresis (2-DE) followed by western blot analysis (WB) with artificial antiserum. A total of 105 Haemaphysalis longicornis (H. longicornis) ticks were selected to prepare protein samples. Some of them were used for 2-DE, and the others were used to immunize mice to collect tick antiserum for WB. Then, 2-DE followed by WB and MALDI-TOF was carried out to screen and identify tick antigens. We identified 19 protein spots representing 12 ORFs: 4 from ticks and 8 from cattle. Among the the 4 ORFs, Tropomyosin, Elongation factor 1-alpha (EF-1α) and Protein Disulfide Isomerase-2 (PDI-2) have been reported as antigens and even vaccine candidate proteins in ticks or other pathogens but not in H. longicornis. Few reports have described dual specificity phosphatase 3 (DUSP-3) in parasites as an antigen. We successfully screened four potential antigens and found that DUSP-3 is a potential novel antigen for ticks.
 

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Pakistan Journal of Zoology

August

Vol. 52, Iss. 4, Pages 1225-1630

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