Submit or Track your Manuscript LOG-IN

Proteomic Analysis by Shotgun LC-MS/MS of Cyst Wall Protein Components From the Protozoan Parasite Cryptocaryon irritans, the Causative Agent of White Spot Disease in Marine Fish

Proteomic Analysis by Shotgun LC-MS/MS of Cyst Wall Protein Components From the Protozoan Parasite Cryptocaryon irritans, the Causative Agent of White Spot Disease in Marine Fish

Honghua Wang1,2, Summia Perveen1,2, Shucheng Shao1,2, Kun Wang1,2 and Fei Yin1,2,*

1Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Collaborative Innovation Centre for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo 315211, People’s Republic of China
2School of Marine Sciences, Ningbo University, 169 South Qixing Road, Ningbo 315832, People’s Republic of China

*      Corresponding author: feige895@gmail.com

 

ABSTRACT

To illustrate the protein composition of the Cryptocaryon irritans cyst wall, a shotgun-liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique was applied and all identified proteins were categorised using a bioinformatics approach. The results showed that 3,472 proteins were identified with a theoretical molecular weight of 11.53–610.90 kDa and an isoelectric point of 4.13–11.77. A total of 63.07% or 50.09% proteins were annotated by Kyoto Encyclopaedia of Genes and Genomes (KEGG) or Gene Ontology (GO) terms, respectively. These results highlight that mass material is transported and catabolised and growth- and death-related proteins are highly expressed, including a number of vesicle-mediated transport, cytoskeletal and extracellular secretion-related proteins. These results provide a platform to study tomont cell characteristics and provide a further understanding of cyst wall formation and mechanisms.
 

To share on other social networks, click on P-share. What are these?

Pakistan Journal of Zoology

April

Vol. 52, Iss. 2, Pages 425-824

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe