Malnutrition is a rising issue in most of the developing countries. To overcome this problem, it is necessary to explore alternate methods for production of non-conventional proteins utilizing the available cheap sources. The indigenous Saccharomyces cerevisiae were isolated from different fruit samples, identified by conventional polymerase chain reaction (PCR) and were characterized for production of single cell microbial protein (SCMP). Out of 60 different fruit samples, a total number of confirmed S. cerevisiae isolates were 1, 2 and 1 from orange (Citrus sinensis), grapes (Vitis), and peach (Prunus persica), respectively. Utilizing yeast extract, peptone and dextrose maximum biomass was produced by S. cerevisiae isolate recovered from peach (SCP-1) i.e. 4.83 g/l. Using isolate SCP-1 higher biomass of S. cerevisiae was produced on sugarcane molasses (6.42 g/l) followed by orange peels. Microbial biomass produced on the sugarcane molasses was rich in crude proteins (56 %) followed by that from orange peels. Highest percentage of alanine (24.72%) and aspartic acid (17.83% and 14.57%) were observed in dried single cell microbial protein produced on sugarcane molasses, orange peels and apple waste, respectively. Soybean meal protein source in broilers ration was replaced by SCMP. The level of antibody titre against Newcastle Disease Vaccine was significantly higher (p≤0.05) than all treated groups. Higher antibody titre was recorded for group C (6g/Kg) (HI≥1:64) followed by group B (4g/Kg) (HI≥1:32) and Group A (2g/Kg) (HI≥1:8) while antibody titre was lower for control group D. Replacement of soybean meal with SCMP did not affect the level of liver enzymes. All the tested liver enzymes were observed to be present in the normal range in all the treated groups A, B, C and D. Feed conversion ratio was not affected in the treated groups and an average FCR of 1.6 was observed in all groups. From the present study it is concluded that indigenous S. cerevisiae can be isolated from different sources and can be used as a source of SCMP in broilers ration to enhance their performance, immunity against pathogenic microbes for production of quality meat.