High blood cholesterol, lipoproteins like low density lipoproteins, very low-density lipoprotein and decreased high density lipoprotein levels are the major risk factors involved in the development of cardio vascular diseases. Lovastatin is formed as secondary metabolic intermediate initiated by anion moiety (acetate) through polyketide chain reactions. The current work was put away to investigate the potency of Pleurotus strains for statin formation by cultivating them on lignocellulosic substrates (wheat straw, rice straw, banana stalk) in SSF for 8 days. Among Pleurotus species (P. nebrodensis, P. sepidus, P. spodoecus), P. spodoecus expressed best statin formation i.e., 9.1±1.4 mg/g. Four physical parameters including temperature, pH, inoculums size and fermentation time (each at five levels) were optimized using central composite design (CCD) design in 30 triplicate experimental runs. The maximum statin formation (19 mg/g) was achieved at 25oC, pH 5.5, inoculums size 3.5 mL and fermentation time 144 h on wheat straw as a growth substrate. The growth medium has an important effect on lovastatin production. Effect of carbon and nitrogen supplementation was investigated due to their restrictive ingredients role. Best yield (49.3±15 mg/g) was achieved by P. spodoecus using lactose (Carbon source) and NaNO3 (Nitrogen source) under pre-optimize circumstances. Different C/N ratios were used to obtain the optimal ratio. Best statin production was attained at 25:1 C/N ratio that was 65.7 ± 5.5 mg/g by P. spodoecus using solid state fermentation.