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Prevalence of Mycoplasma gallisepticum in Poultry and Wild Life Birds Suspected of Chronic Respiratory Disease in Northern Pakistan

Prevalence of Mycoplasma gallisepticum in Poultry and Wild Life Birds Suspected of Chronic Respiratory Disease in Northern Pakistan

Nasir Abbas1, Muhammad Suleman1, Nazir Ahmad Khan2,*, Ijaz Ali3, Mubashir Rauf1,5 and Sadeeq ur Rahman4,*

1Department of Microbiology, Hazara University, Mansehra 21300, Khyber Pakhtunkhwa
2Department of Animal Nutrition, The University of Agriculture, Peshawar
3Department of Biosciences, Bioscience Block, Chak Shehzad Campus, Park Road, COMSATS Institute of Information Technology, Islamabad
4College of Veterinary Sciences and AH, Section Microbiology, Abdul Wali Khan University, Mardan, Khyber Pakhtunkhwa
5College of Veterinary Sciences, Bahauddin Zakariya University, Sub Campus Layyah

*      Corresponding authors: sadeeq@awkum.edu.pk; nak126@gmail.com

 

 

Fig. 1.

Detection and isolation of M. gallisepticum. Colonial morphology of M. gallisepticum is shown with left of 7 days post incubation, while, the right one was of 15 day post inoculation (c and d) Frequency (percentage) of detection of M. gallisepticum among isolates obtained after culturing swab samples on specific media (b) PCR-based detection of M. gallisepticum recovered from CRD suspected birds. Specie specific primers were used on the samples to amplify 185 bp long PCR product as shown by the arrow. The PCR amplicons are resolved on 1% agarose gel. The size was anticipated from a DNA ladder run in parallel. M, marker; C, positive control (DNA isolated from commercially available M. gallisepticum strain used for vaccination); w, water (genomic DNA was replaced with water for negative control); x, master mix and genomic DNA obtained from E. coli without water primers.

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Pakistan Journal of Zoology

June

Vol. 50, Iss. 3, Pages 799-1198

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