Submit or Track your Manuscript LOG-IN

Prevalence of Extended Spectrum β-lactamase SHV and OXA Producing Gram Negative Bacteria at Tertiary Care Hospital of Lahore, Pakistan

Prevalence of Extended Spectrum β-lactamase SHV and OXA Producing Gram Negative Bacteria at Tertiary Care Hospital of Lahore, Pakistan

Abdul Waheed1,2, Sidrah Saleem1, Naveed Shahzad3, Junaid Akhtar4, Muhammad Saeed5, Iqra Jameel1, Farhan Rasheed6 and Shah Jahan7,*

1Department of Microbiology, University of Health Sciences, Lahore, Pakistan
2College of Allied Health Professional, Directorate of Medical Sciences, Government College University, Faisalabad, Pakistan
3School of Biological Sciences, University of the Punjab, Lahore, Pakistan.
4Department of Allied Health Sciences, Sargodha Medical College, University of Sargodha, Sargodha, Pakistan
5Pathology Department, DHQ Hospital, Mandi Bahauddin, Pakistan
6Department of Microbiology, Allama Iqbal Medical College, Lahore, Pakistan 7Department of Immunology, University of Health Sciences, Lahore, Pakistan.

*      Corresponding author: shahjahan@uhs.edu.pk

 

ABSTRACT

The present study was planned to detect drug resistant genes like “blaSHV” and “blaOXA” among gram negative rods (GNRs) isolated from different clinical specimens, collected from Jinnah Hospital Lahore during January-December 2015. Majority of the GNRs were isolated from urine samples (55%) followed by pus (28%), blood (7%), sputum (6%), Broncho-alveolar lavage (2%), fluid (1%) and from tips (1%). The isolated organisms were Escherichia coli (n=60), Klebsiella pneumoniae (n=20), Enterobacter cloaca (n=09), Enterobacter aerogenes (n=02), Proteus mirabilis (n=05), Proteus vulgaris (n=02) and Citrobacter freundii (n=02). Extended spectrum beta lactamases (ESBL) production was screened by double disc synergy test (DDST) and combination disc method as per Clinical Laboratory Standard Institute (CLSI) guidelines 2015. Presence of “blaSHV” and “blaOXA” genes was tested by PCR technique. Every isolate was ESBL positive and resistant against third generation cephalosporin and Aztreonam. Notably, 15% of ESBL producing strains harbored blaSHV gene while 43% were positive for blaOXA gene. In a nutshell, diverse variety of ESBL-positive GNRs is distributed, the findings of the present study should be considered for planning strategies to treat and prevent the further spread of ESBL-producing gram-negative rods infections.
 

To share on other social networks, click on P-share. What are these?

Pakistan Journal of Zoology

December

Vol. 51, Iss. 6, Pages 1999-2399

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe