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Potential Recruiting and Hepatoprotective Effects of Ellagic Acid in D-Galactosamine-Induced Liver Damage in Rats

Potential Recruiting and Hepatoprotective Effects of Ellagic Acid in D-Galactosamine-Induced Liver Damage in Rats

Mustafa Cengiz1*, Jama Hussein Ali2, H. Mehtap Kutlu2, Djanan Vejselova2 and Adnan Ayhanci3

1Department of Elementary Education, Faculty of Education, Siirt University, Siirt, Turkey
2Department of Biology, Faculty of Science, Anadolu University, Eskisehir, Turkey
3Department of Biology, Faculty of Arts and Science, Eskişehir Osmangazi University, Eskisehir, Turkey

*      Corresponding author: mustafacengizogu@gmail.com

Fig 1
Histological structure of rat liver. Group 1, the control, saline treatment; Group 2, 0.2% DMSO treated liver tissues; Group 3, tissues treated with GalN; Group 4, EA administered liver tissues before GalN treatment; Group 5, tissues administered with GalN before EA. (inf, inflammatory; *, vascular congestion; , cellular damage; ve, venul; a, arteriole; s, bile duct 50 µm (x40) HE, haematoxylin and eosin stained rat liver tissues.
Fig 2

Transmission electron micrographs showing morphological changes in rat liver cells. A, Group 3-GalN treated tissue showing the nucleus (N) compressed by large lipid droplets (L). The cytoplasm shows fewer rER and glycogen rosettes in-between high electron-dense mitochondria (m) and dilated sER (S) (4200x); B, Group 4-Tissues EA administered before GalN treatment with showing less lipid droplets (L), less electrondensity of mitochondria (m), less dilated sER (S) and condensed glycogen rosettes (G) compared to group 3 (8200x); C, Group 5- GalN before EA administered liver tissue with showing fewer lipid droplets (L) and ring shaped chromatin condensation © compared to D-galactosamine group (4200x).

Fig 3

Immunohistochemically-stained liver specimens of the rats in different study groups. Activated caspase-3 and Bax in control group and 0.2 % DMSO-treated showing almost negligible staining normal hepatocytes (B, C, E and F) while Bcl-2 protein expression in control group showing less intense staining normal hepatocyte as shown by arrows (A and D). Caspase-3 and Bax protein expression in D-GaIN-treated group showing more intense staining of hepatocytes and diffused staining as shown by the arrows (H and I) while Activated Bcl-2 in D-GaIN-treated group showing almost negligible staining normal hepatocytes as shown (G). Activated Caspase-3 and Bax immunostaining of liver treated with EA before D-GaIN and D-GaIN before EA showing less intense staining of hepatocytes as shown by arrows (K, L, N and O) while Bcl-2 protein expression in EA before D-GaIN and D-GaIN before EA groups showing less intense staining of hepatocytes as shown by arrow (J and M) (bar: 50µm).

Fig 4

Intensity of immunolabelling score of activated Bcl-2 (A), caspase-3 (B) and Bax (C) positive hepatocytes in the groups. **p<0.001 and *p<0.05compared to control (group 1) and 0.2% DMSO groups (group 2).

Pakistan Journal of Zoology (Associated Journals)

June

Vol. 49, Iss. 3, Pages 761-1149

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