Submit or Track your Manuscript LOG-IN

Potential of Microalgae as Feed Supplements for Sustainable Aquaculture


Potential of Microalgae as Feed Supplements for Sustainable Aquaculture

Irshad Ahmad1,2*

1Department of Bioengineering, King Fahd University of Petroleum and Minerals, Dhahran 31261, Kingdom of Saudi Arabia.

2Interdisciplinary Research Center for Membranes and Water Security, King Fahd University of Petroleum and Minerals, Dhahran 34464, Kingdom of Saudi Arabia.


Aquaculture is a fast-growing industry mainly depends on the key feedstuffs, fishmeal (FM) and fish oil (FO) that will be limited with the passage of time due to the insubstantial resources available for wild fish harvesting. Therefore, other sources of feedstuffs need to be investigated to substitute FM and FO in aquafeeds. Terrestrial crops can be used to substitutes a portion of the FM however; they can result in changes in the nutritional quality of the fish produced. Microalgae can be considered as a favorable alternative that can substitute FM and FO ensuring the principles of sustainability in aquaculture. Microalgae are reasonably rich in proteins, lipids, carbohydrates, vitamins, minerals, pigments, etc., which are essential for not only sustaining fish health but also its unique array of bioactive compounds can improve coloration and quality of fillet. The aim of this review is to provide an update of the current knowledge of microalgae as a supplement or feed additive to substitute FM and FO in aquafeeds. This review will provide a platform to highlight the potential of microalgae-based aquafeeds for a sustainable aquaculture industry.

Article Information

Received 24 February 2022

Revised 18 May 2022

Accepted 25 June 2022

Available online 15 July 2022

(early access)

Published 14 November 2022

Key words

Marine phytoplankton, Aquatic species, Fish meal and oil, Microalgae aquafeeds, Microalgae nutrients, Circular biorefinery


* Corresponding author:

0030-9923/2023/0001-419 $ 9.00/0

Copyright 2023 by the authors. Licensee Zoological Society of Pakistan.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (


Aquaculture is an important and fast-growing food sector in the world playing a vital role in human’s life. It has undoubtedly contributed significantly to improve the nutritional value of human’s diet in the form of seafood and improved the standard of living due to financial gain from the rapidly developing aquaculture industry. Since 2016, worldwide fishery and aquaculture foods have shown tremendous growth of ~ 171 million tons that costs 194.78 billion euros through aquaculture to give 54.5% of the overall production. Statics shown that 19.3 million people have obtained employment in the fisheries and aquaculture sector that accounts 30% of the total jobs, playing a significant role in uprising their socio-economic status. The entire fish production without marine floras is likely to upsurge ~ 204 million tons in 2030 that accounts an overall increase of 15 % over 2018 (FAO, 2018, 2020).

Aquaculture is a vital source of animal protein, which is nearly half of the total production that need outward feed contributions to overcome the food consumption demand by the growing human population. Aquafeeds normally contain fishmeal and oil take out from trivial pelagic forage fish, for instance sardines, herrings and anchovies, and little amount after fish embellishments and castoffs. Fish meal (FM) and fish oil (FO) mainly used to fulfil the protein and fatty acid requirements of farmed aquatic species as palatable and inexpensive feed components. Aquafeeds generally increases fish productivity, but an alternative to FM and FO must be find out for sustainable fish farming (FAO, 2016; Turchini et al., 2020). Many fodder fisheries are fully or over exploited, and reports have portrayed the existing trend of fishmeal and oil utilization as a big challenge for marine biodiversity and human food safety (Froehlich et al., 2018). The current fishmeal and oil are very costly, expected to upswing than that of plant oils and protein meals over the following decade. Keeping in mind all issues alternative aquafeed resources are required with high digestibility and nutritional cost analogous to FM and FO that needs to be developed through eco-friendly strategies as extraordinary and economical feedstuffs (Cottrell et al., 2020).

Marine-based FO contain high quantity of omega-3 (n-3) long-chain (≥C20) polyunsaturated fatty acids (LC-PUFAs), namely eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3). Biological conversion of the PUFA, alpha-linolenic acid (ALA; 18:3n-3), normally found in terrestrial oil kernels of canola used in marketable salmonid nourishments, to EPA and DHA in fish and humans is not sufficient to fulfil their nutritional rations, therefore it is necessary to take in the n-3 LC-PUFA in food (Saini and Keum, 2018; Bou et al., 2017). DHA play an essential role in cell signaling as well as the structure, function, and fluidity of cell membranes while EPA triggers anti-inflammatory response via eicosanoid production. Addition of EPA and DHA in salmonid feed not only safeguard ample growth and development of the fish, but also a carrier to deliver these EFAs to humans, which have significant health benefits in preventing rheumatoid arthritis, cardiovascular, and neurological ailments (Hart et al., 2021; Siscovick et al., 2017; Laye et al., 2018).

The current aquaculture is over relied on terrestrial crops and animal-based materials like soybean meal, canola oil, poultry fat, and blood meal, which involves worries about deviation of crops and animals from human feeding towards aquafeed (Colombo, 2020). As crops growing sector appearances a universal task to nourish nearly a billion of hungry folks, and risks turning the rapidly expanding aquaculture sector into an environmentally unsustainable agrarian practice for the world’s grains and oils consumption. Their use in aquafeed has many shortcomings e.g., unbalanced essential amino acid, high levels of antinutritional elements and insufficient level of EAA and EFA cannot fulfil the requirements of fish and human health (Fry et al., 2016; Sprague et al., 2016).

Microalgae are eukaryotic photosynthetic microorganisms that use solar energy, nutrients, and carbon dioxide (CO2) to produce proteins, carbohydrates, lipids, and other valuable organic compounds. Recently an increasing attention focused all over the world on commercial-scale production of microalgae for aquaculture feeds due to their better fatty acid profiles. Compared to terrestrial plant proteins and oils, microalgae have reasonable quantity of DHA and EPA (Acquah et al., 2020). They can propagate under different conditions (autotrophic, heterotrophic and mixotrophic) by assimilating simple nutrients and accumulate useful metabolites like n-3 LC-PUFA and carotenoids (Hardwood, 2019).

Microalgae can deliver many vitamins specially vitamins D and K produced in little quantity in the land-dwelling plants. This insufficiency can be fulfilled by adding microalgae in the aqafeed that can also provide other vitamins (A, B, C, D, and E) (Del Mondo et al., 2020; Kiran and Mohan, 2021). In a study, Arthrospira platensis and Chlorella vulgaris were used in the aquafeed to replace fishmeal given to post-larvae of freshwater prawn (Macrobrachium rosenbergii) to investigate its influence on vitamin C and E, antioxidant potential, catalase, and lipid peroxidation activities. After 3 months, a 50% substitute of the fishmeal with A. platensis has significantly enhanced the growth of M. rosenbergii (Radhakrishnan et al., 2016). Recently the total folate content was determined in different species of marine microalgae. The marine microalgae Picochlorum sp. showed the highest folate content (6,470 ± 167 µg/100 g dry biomass), followed by Chlorella vulgaris (3,460 ± 134 µg/100 g dry biomass), and other tested strains (Woortman et al., 2020). In another study among seven microalgae species cyanobacterium (Anabaena cylindrical) was find out as a rich source of vitamin K1 producing 200 μg g−1 on a dry-weight basis, which is about six-fold greater than its rich dietary sources (spinach and parsley) and can be further increased by optimizing the growth conditions (Tarento et al., 2018).

Microalgae strains display a tremendous variation in the inorganic content (ash) or mineral composition due to their existence in diverse habitats, wide-ranging environmental factors, and different genetic composition. The minerals content in microalgae varies from 20-40% that play a significant role in the structural, physiological, catalytic, and regulatory functions of the aquatic organisms (Fox and Zimba, 2018). In a study, the micro and macro minerals of five maritime microalgae strains showed capricious ranges in calcium, phosphorus, magnesium, potassium, sodium, and sulfur as 0.26-2.99, 0.73-1.46, 0.26-0.71, 0.67-2.39, 0.81-2.66, and 0.41-1.38%, respectively. The chlorophyte (Tetraselmis chuii) showed the highest level of calcium and phosphorus as 2.99 and 1.46%, respectively. Similarly, the bacillariophyte (Phaeodactylum tricornutum) showed highest level of magnesium, potassium, sodium, and sulfur content respectively. Recently the mineral conformation was investigated in maritime microalgae comprised 26 chemical elements (Al, B, Ba, Be, Bi, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, Sn, Sr, Ti, Tl, V, and Zn). The tested strains showed a high inorganic content ranges from 12.9-36.3% mass of the examined elements per dry biomass (Tibbetts et al., 2015; Silva et al. 2015). It is also useful for a sustainable aquaculture industry through blue revolution by the minimum use of water, farming land, nutrient recycling, CO2 conversion, and remediation of wastewater (Tibbetts, 2018; Yarnold et al., 2019). Consequently, microalgae-aquaculture association is an emerging paradigm for sustainable aquaculture production that will ultimately shift the aquaculture industry into an ecofriendly circular bioeconomy.


Microalgae are very diverse group of eukaryotic photosynthetic organisms usually existed in marine and freshwater environments (Daneshvar et al., 2018). They can propagate in different forms such as single cells or in chains or in the form of trivial clusters (Postma et al., 2016), and play a vital role in marine environment by utilizing the sunlight and CO2 for the synthesis of different biomolecules like proteins, polysaccharides, lipids, vitamins, or pigments (Ibrahim et al., 2020; Moha-Leon et al., 2018). Consequently, they play an important role in nourishing the trophic chains in the aquatic environments and world widely distributed with >7000 species in diverse environments (Bellou et al., 2014; Shah et al., 2016).

Microalgae are the principal food source by providing necessary nutrients to the zooplankton and lower to higher trophic fish in the food chain (Yarnold et al., 2019). Different microalgae species comprise up to 60% protein, 60% carbohydrates or 70% oils based on the specificity of the strain and respective growth conditions (Draaisma et al., 2013) and contain valued pigments, growth hormones and secondary metabolites with substantial antimicrobial, antioxidant, anti-inflammatory and immunostimulant characteristics that are very beneficial for aquatic organisms (Garcıa-Chavarrıa and Lara-Flores, 2013; Shah et al., 2017). Consequently, microalgae can be incorporated in aqafeeds to nourish the fish larvae, mollusks, and crustaceans. It can be also used as live food to feed the zooplanktonic organisms like rotifers and micro-crustaceans (Copepod, Cladocera and Artemia sp.) that are live prey of maritime and crustacean larvae (Conceicao et al., 2010; Hemaiswarya et al., 2011; Perez-Legaspi et al., 2018; Yarnold et al., 2019). Microalgae is a balanced feed source of protein, lipid, and carbohydrate suitable to protect fish health. Table I shows the nutritional content of microalgae compared with other alternative feed ingredients.


Table I. Nutritional content of alternate feeds.

Feed ingredient

Protein (%)

Lipid (%)

Carbohydrate (%)


Wheat meal




Sørensen et al., 2011

Soybean meal




El-Sayed, 1994

Corn-gluten meal




Liu et al., 2020

Fish meal




Hodar et al., 2020

Saccharomyces cerevisiae




Blomqvist et al., 2018

Hermetia illucens




Varelas, 2019

Hydrolyzed feather meal




Yu et al., 2020





Madeira et al., 2017





Madeira et al., 2017

Botryococcus braunii




Tavakoli et al., 2021

Dunaliella sp.




Madeira et al., 2017

Spirulina maxima




Madeira et al., 2017

Spirulina platensis




Madeira et al., 2017





Samuelsen et al., 2018

Porphyridium aerugineum




Madeira et al., 2017

Phaeodactylum tricornutum




Sørensen et al., 2016

Chlorella vulgaris




Viegas et al. 2021

Chlorella sorokiniana




Guldhe et al. 2017

Anisancylus obliquus




Ansari et al. 2021

Scenedemus obliquus




Viegas et al. 2021 NA

Pavlova sp.




Madeira et al., 2017

Nannochloropsis granulata




Tibbetts et al., 2017

Isochrysis galbana




He et al., 2018



The microalgae biomass contain protein as an important component and its yield is dependent on many aspects e.g. type of specie, growth circumstances (pH, light, and temperature), nutritive value and environmental conditions. Nitrogen is an essential element to increase the protein yield of microalgae. A higher quantity of protein has been reported in microalgae when grown in high nitrogen concentration. The aquaculture industry uses ~70% of high-protein aquafeeds for the enhanced growth of aquatic organisms (Ansari and Gupta, 2019; Hua et al., 2019). The aquafeeds usually contain high quantity of protein with all the required amino acids however, majority of the current aquafeeds based on terrestrial plant protein are missing in some of the essential amino acids for instance lysine, methionine, threonine etc. It has been reported that microalgae contain virtually all the required amino acids therefore instead of terrestrial plants inclusion of microalgae in the aqafeed can produce more nutritious aquatic organisms that will be beneficial for human health (Chrapusta et al., 2017).

According to the recommendations of WHO/FAO/UNU vis-a-vis humans body need for essential amino acids the microalgae species (Chlorella and Arthrospira) contain high quality proteins and their amino acids profiles are almost similar to the protein sources (eggs and soybean) (Chronakis and Madsen, 2011). Microalgae can be added as feed or feed additives in the prospective aquafeed formulation of fish, shrimp, crab, shellfish, sea cucumber and other aquatic organisms. Microalgae was grown in an outside raceway reactor provided with digestate that was partially substituted (10% of the diet) in aquafeed of the Acipenser baerii. The outcomes of the experiments have confirmed the practicability to grow microalgae on digestate shown higher yield (6.2 gDM m−2 d−1) with enhanced nutrient removal and reducing the chemical oxygen demand. The feeding test of the experiment compared with control groups (p > 0.05) shown better growth recital, somatic directories, fillet nutritional configuration and celiac function point out the significance of microalgae as protein source could be used in Siberian sturgeon aquafeed (Bongiorno et al., 2020).


Microalgae are a rich source of lipids that represents 74% of microalgae’s total biomass depends on the species (Bernaerts et al., 2019). The lipids are made of fatty acids by 12-24 carbon atoms that comprise polyunsaturated fatty acids of n-3 PUFAs and n-6 PUFAs families, respectively (Patras et al., 2019). Microalgae produces eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) that are vital for the growth, reproduction, immunity, and nutritional value of aquafeed (Remize et al.2021). Many researchers are currently investigating the effectiveness of microalgae that can be developed as non-FM or -FO diets. In one of such study fish feed and oil was substituted by algae feed (Schizochytrium sp. powder) and plant proteins. The results imply the potential of microalgae as an alternative source of Omega-3 fatty acid proceeding marine fish (Oliver et al., 2020). Currently the nutritional digestibility of a maritime microalga (Schizochytrium spp.) enriched in DHA and LC-PUFA was determined as an alternative lipid source fed to rainbow trout (Oncorhynchus mykiss). The results demonstrated the ADCs of the nutrients, energy, DHA and other fatty acids profiles of Schizochytrium spp. as an excellent alternative of fish oil replacement that may be provided with extra LC-PUFA in fish feed with vegetable oils (Bélanger et al., 2021).

Microalgae embody an auspicious prospect of Omega-3 PUFA yield, and several species have the capability to naturally synthesize EPA in high quantity such as Nannochloropsis, Phaeodactylum tricornutum, Odontella aurita, Monodus subterraneus, and Pophyridium cruentum (Bernaerts et al., 2018). The EPA levels (12.74 ± 1.84% and 10.93 ± 1.84%) have been reported in Nannochloropsis salina CCMP1176 and Nannochloropsis oceanica CCMP537 proceeding total fatty acids (Ma et al., 2014). Similarly, in another study, EPA levels (22.4 - 31.4 ± 1.7%) was reported in Phaeodactylum tricornutum (Ryckebosch et al., 2014). At present, numerous enterprises are producing EPA-rich microalgae biomass under photoautotrophic conditions such as Shenzhen Qianhai Xiaozao Technology Co., Ltd. of China harvests EPA rich lipid extract from Nannochloropsis salina. Additional companies are Arizona Algae Products, LLC (US) or Simris Alg AB (Sweden) (Oliver et al., 2020). Currently microalgae (Aurantiochytrium sp.) which as key source of n-3 PUFAs has been investigated to determine its potential on the growth performance and immune response of Trachinotus ovatus, supplied with different microalgae content (1.00-11.00%) for 8 weeks in its diets. The results revealed that adding Aurantiochytrium sp., in the diet has a beneficial paraphernalia on the fish survival, weight gain, and explicit growing rate improved by 1.02, 1.16, and 1.08 times, respectively (Li et al., 2021).


Aquaculture is the rapidly growing industry faces the problem of disease outbreaks usually controlled by traditional methods of using antibiotics and chemical disinfectants; however, they have the problems of resistance development and bioaccumulation of toxic residues in aquatic organisms. Vaccines are an effective way of disease control, but its use is time consuming, expensive, and traumatic to the fishes. Therefore, immunostimulants are natural compounds that trigger the host defense system against infections. Currently microalgae polysaccharides are used as immunostimulants to control the diseases in aquatic organisms have been focused due to its less toxicity, bioactivity, and environment friendly nature (Marudhupandi and Inbakandan, 2015). The bioactivities and applications of sulfated polysaccharides from microalgae has been reported as anti-inflammatory, immunomodulatory, antiviral and antioxidant properties in the aquatic organisms (Raposo et al., 2013; Amna et al., 2018; Mohan et al., 2019; Nastasia et al., 2020). In a recent report, sulfated polysaccharides have been isolated from Codium fragile that have immuno-stimulatory effects on Olive flounder and can be utilized as feed additive to heighten the immunity of fish (Yang et al., 2019).

Microalgae can be used as nutritional supplements in the aquafeeds for their potent immuno-stimulatory effects in aquatic organisms. Currently it was observed that supplementing C. vulgaris at 10% in the meal of O. niloticus has protected it beside arsenic-induced immunotoxicity and oxidative stress (Zahran et al., 2018). Similarly, adding C. vulgaris at 6% in the meal of gigantic freshwater prawn (M. rosenbergii) has shown enhanced prophenol oxidase activity with the entire quantity of hematocytes of M. rosenbergii post larvae that might improve the larval survival to Aeromonas hydrophila infection (Maliwat et al., 2017). In another study C. vulgaris was added as dietary supplementation of nile tilapia (O. niloticus) to protect it against sub-lethal concentrations of penoxsulam herbicide and improve its anti-infective capacity against Aeromonus sobria (Galal et al., 2018). Similarly, the dietary intake of 5% Schizochytrium limacinum has encouraging results in improving the intestinal health and nutrient utilization potential of rainbow trout O. mykiss (Lyons et al., 2017).


Microalgae produce carotenoids with distinctive antioxidant and coloring characteristics including xanthophylls e.g., zeaxanthin, lutein, antheraxanthin that are found in land-dwelling plants. Moreover, they can also produce other pigments (astaxanthin, fucoxanthin, diatoxanthin, diadinoxanthin) specifically found in algae, cyanobacteria, and some species of yeast (Novoveská et al., 2019; Ambati et al., 2014, 2019). Numerous carotenoids are used in the aquaculture industry to color farmed fish especially astaxanthin is utilized to augment the pigmentation in farmed salmon. The pigmentation of fish is an important factor that can stimulate the consumer’s choice to buy it. Carotenoids are not only important for coloring but also show a significant role in the growth, reproduction, and health care of aquatic organisms (Alfnes et al., 2006; Lehnert et al., 2019; Costa and Miranda-Filho, 2019).

The aquatic animals are unable to synthesize carotenoids therefore, microalgae can be provided as feed additive in their meal which are their naturally producers. An important carotenoid astaxanthin that is commercially used in the aquaculture industry produced by a microalga (Haematococcus pluvialis) at>4 percentage per DW that is a promising yield as compared to other organisms (Butler et al., 2018). Spirulina was added as a carotenoid source (0, 2.5, 5, and 10% of fishmeal weight) in the feed of yellow tail cichlid Pseudotropheus acei. The data shows a significant increase in total eggs production, percentage of eggs hatching, enhanced growth rate and raised carotenoids level in the skin of experimental one as compared to the control group of fishes (Güroy et al., 2012). In a study, four fish meals were supplemented with the carotenoids (astaxanthin, lutein, canthaxanthin and lutein+canthaxanthin) standardized at 50 mg kg-1 in the diet of goldfish juveniles compared to control (without carotenoids). The meal with lutein, astaxanthin and canthaxanthin showed a greater persistence values and increased carotenoid pigmentation if the skin of goldfish juveniles as compared to control treatments (Besen et al., 2019).


The aquaculture production has tremendously increased during the last decade due to the amassed consumer’s demand. Hence, this sector needs massive quantities of aquafeed that depends on FM, FO, and terrestrial plants, problems of low nutrients status, less availability, and expansive. To overcome these issues, microalgae is the best economical and alternative feed ingredient in aquafeed. Currently live microalgae strains as a whole or lipid-extracted algae (LEA) have been tried in aquafeed that have significantly enhanced the growth performance, physiological movement, and nutritional status of the aquatic species (Ansari et al., 2021). Numerous studies have been conducted to determine the potential substitution of conventional constituents in aquafeed with microalgae as shown in Table II.


Table II. Studies conducted to determine the potential substitution of conventional constituents in aquafeed with microalgae.

Microalgae species + Aquatic species

Ingredient substituted

Effects of microalgae specie on the growth performance and feed utilization of aquatic species


1. Nannochloropsis sp. + Dicentrarchus labrax


Nannochloropsis sp. partially substitute 10% of the diet has no adverse effects on the growth performance, dietary nutrient consumption, and gut enzymes

Pascon et al., 2021

2. Schizochytrium sp. + Oncorhynchus mykiss


Microalga can be a better candidate to substitute FO and LC-PUFA in FM due to improved ADCs of the nutrients, energy, DHA and other fatty acids

Bélanger et al., 2021

3. Chlorella vulgaris + Macrobrachium rosenbergii


Adding 4-8% chlorella as a replacement of FM significantly enhanced the explicit growth rate, immune response, and resistance of M. rosenbergii postlarvae counter to Aeromonas hydrophila pathogen

Maliwat et al., 2021

4. Schizochytrium sp. + Salmo salar


Microalgae biomass was added as 30% in diets of S. salar that specify the Sc biomass as an extremely digestible source of DHA and protein

Hart et al., 2020

5. Chlorella sp. + Cyprinus carpio


Fresh microalgae that performed well in nutrient assimilation and oxygen production replaced FM, reducing eutrophication and providing O2 in aquaculture.

Chen et al., 2020

6. Schizochytrium sp. + Oreochromis niloticus


Microalga has modulatory effects on the blood cells and celiac microorganisms, without disturbing the configuration and integrity of intestinal villi

Souza et al., 2020

7. Scenedesmus-chroococcus + Acipenser baerii


Microalgae supplemented diet accomplishes the nutrient necessities, confirming appropriate growth, ample fillet quality and a vigorous gastrointestinal tract in fish

Bongiorno et al., 2020

8. Nannochloropsis oculata and Schizochytrium sp. + Oreochromis niloticus

FM and FO

FM and FO replaced with two microalgae species in fishmeal has produced highest amount of DHA in the fillet than in those fed conventional feed recommends a cost effective aquafeed for farmed fish

Sarker et al., 2020a

9. Nannochloropsis sp., Isochrysis sp., and Schizochytrium sp. + Oncorhynchus mykiss

FM and FO

Microalga showed better results to substitute FM and FO due to improved ADCs of the crude protein, amino acids, lipid, and other fatty acids

Sarker et al., 2020b

10. S. obliquus + Oreochromis niloticus


In different microalgae-based FMs, the diet comprising 7.5% of whole and LEA deliver essential nutrients with significant growth performance indicators (FCR 1.36 g/g, PER 1.84 g/g, and HSI 2.01%) in O. niloticus

Ansari et al., 2020

11. N. oceanica + Anarhichas minor


FM of A. minor may be substituted up to 15% of the N. oceanica rich in omega 3-fatty level improved in the fish body

Knutsen et al., 2019a

12. S. obliquus + Anarhichas minor


Substituting 4% of FM has significant impact on the body weight from 140 to 250 g after 12 weeks with rapid muscle growth, proximate arrangement of muscle, and skin color of fish

Knutsen et al., 2019b

13. Haematococcus pluvialis + Perca flavescens


LEA meal mixed by soy protein (10% of the diet) replace 25% of FM in the tested diet has no antagonistic paraphernalia on the growth performance with growth indicators (FCR 1.19 g/g, PER 1.76 g/g, and HSI 2.00%) compared to the control diet

Jiang et al., 2019

14. Gracilaria arcuate + Oreochromis niloticus


FM of O. niloticus replaced with 20% G. arcuata has tremendously increased their body weight from 13.01 to 36.13 g after 12 weeks with the growth indicators (FCR 2.28 g/g, and PER 1.49 g/g)

Younis et al., 2018

15. Nannochloropsis oculata + Oreochromis niloticus


Substitution of FM with 33% LEA showed improved growth performance, feed use, and persistence comparable to control diet. After 12 weeks, the body weight increased from 1.98 to 28.06g with the growth indicators (FCR 1.26 g/g, and PER 2.12 g/g)

Sarker et al., 2018

Table continued on next page ...........................

Microalgae species + Aquatic species

Ingredient substituted

Effects of microalgae specie on the growth performance and feed utilization of aquatic species


16. Nannochloropsis granulate + Litopenaeus vannamei


DP Protein content of all N. granulate meals was adequate and can be potentially added in the meals of L. vannamei

Tibbetts et al., 2017

17. Isochrysis sp. + Tridacna noae


Microalgae was used as LF source to determine its consumption and digestion by T. noae larvae that was subjective to the type of microalgae and larval age

Southgate et al., 2017

18. Schizochytrium + Litopenaeus vannamei


Addition of 4% Schizochytrium in diet showed significantly higher specific growth rate in shrimp larvae without effecting their survival, activities of gut enzymes, and fatty acid profile

Wang et al., 2017

19. Schizochytrium sp. + Salmo salar L


Schizochytrium sp. was incorporated as FO in the diet of S. salar that showed enhanced growth performance, high fillet quality, nutrient retention, and blood chemistry

Kousoulaki et al., 2016

20. Haematococcus pluvialis + Seriola rivoliana


FM substituted up to 80% without sizable effect on the intestinal effectiveness of S. rivoliana with an improved body weight from 2.5 to 74.0 g after 9 weeks, and growth indicators (FCR 0.8 g/g, and HSI 1.1%)

Kissinger et al., 2016

21. Desmodesmus sp. + Salmo salar L


Addition of 20% Desmodesmus sp. in FM had no adversative effect on the growth depiction with growth indicators (FCR 0.90 g/g, PER 2.36 g/g, and HSI 1.30%)

Kiron et al., 2016

22. Arthrospira platensis + Macrobrachium rosenbergii


Addition of 50% FM by A. platensis considerably improved their growth, feed efficiency, and enhanced amino acids' proteins and oil content

Radhakrishnan et al., 2016

23. Phaeodactylum tricornutum + Salmo salar L


Addition of 6% microalgae biomass has no undesirable effects on the growth, feed conversion or ADCC of protein, lipid, energy, ash, and DM

Sørensen et al., 2016

24. Schizochytrium sp. + Oreochromis niloticus


Microalgae proved a better substitution of FO in the diet of Oreochromis sp with better weight gain, feed conversion ratio, protein efficiency ratio without effecting its survival rate

Sarker et al., 2016

25. Pavlova viridis and Nannochloropsis sp. + Dicentrarchus labrax


Addition of 50-100% microalgae to replace FO in the diet of Dicentrarchus sp has no negative effects on their growth performance and nutrient utilization

Haas et al., 2016

26. Ulva ohnoi and Entomoneis spp. + Salmo salar L


Two added algal harvests (2.5 and 5.0%) delivered same fish enactments and feed efficacy (rich in n-3 LC-PUFA) related to the reference intake

Norambuena et al., 2015

27. Chaetoceros muelleri and Tisochrysis lutea + Panopea generosa


Compared to the spray-dried, live-microalgae diets showed reasonable protein, carbohydrate, lipid, energy, DHA, n – 6 DPA, and Σn – 3 PUFA content; elevated EPA, AA, and ΣMUFA levels and an elevated Σn – 3/Σn – 6 PUFA ratio

Arney et al., 2015

28. Isochrysis sp. + Dicentrarchus labrax L.

PR and FO

Replacing 20% PR and up to 36% FO by Isochrysis sp. has no adversative effects, feed intake or growth performance as compared to controls

Tibaldi et al., 2015

 29. Arthrospira platensis + Oncorhynchus mykiss


Replacing 10% diet of Oncorhynchus sp with Arthrospira sp has tremendously increased their red and white blood count, hemoglobin, total protein, and albumin levels

Yeganeh et al., 2015


LEA, Lipid-extracted microalgae; FM, Fish meal; FO, Fish oil; LF, Larval food; PR, Protein; DP, Digestible protein; ADC, Apparent digestibility coefficients.


The Nannochloropsis sp. has been used as living food in aquatic species in a high-rate algal pond (HRAP) system on zootechnical basis considering morphometric parameters, and dietary nutrient digestibility of the celiac system of Dicentrarchus labrax. According to the results 10% of terrestrial plant ingredients replaced with microalgae has significantly increased the final body weight of the fish without disturbing its growth performance, dietary nutrient utilization, and gut enzymatic activities (Pascon et al., 2021). Microalga (Schizochytrium spp.) has been investigated to determine its digestibility, macronutrients availability and individual fatty acids (omega 3-rich) in Oncorhynchus mykiss. Thus, the microalgae were found to be a potential ancillary of FO and LC-PUFA in FM (Bélanger et al., 2021). Similarly, Schizochytrium sp. was included as 30% in the diets of S. salar that could reinstate the fillet n-3 LC-PUFA content as well as increase nutritional quality of the product for consumers (Hart et al., 2021). In another study addition of C. vulgaris (4-8%) in diets of Macrobrachium rosenbergii delivered best growth rates and enhanced immunity of the post larvae (Maliwat et al., 2021).

The traditional aquaculture is facing with the problems of eutrophication and depletion of oxygen for the aquatic organisms. In this regard, microalgae can be helpful in providing oxygen during its natural photosynthetic process as well as assimilating the nutrients released from the sludge and providing a conducive environment for the better growth and survival of aquatic organisms (Chen et al., 2020). The Schizochytrium sp. was added in the diet of O. niloticus reared in net cages that has beneficial effects on the red blood cells, lymphocytes, and intestinal microbiota without any contrary effects on the structure and integrity of the intestinal villi (Souza et al., 2020).

Aquaculture companies have focused to reduce the cost of aquafeed by replacing FM and FO by adding sustainable and cost-effective microalgae to produce fish free feed for O. niloticus. Such an effort was carried out by adding two microalgae species to replace FM and FO in the diet of farmed fish, which has beneficial effects such as highest amount of lipid, protein and DHA in the fillet as compared to the conventional feed (Sarker et al., 2020a). Similarly, three microalgae species (Nannochloropsis sp., Isochrysis sp., and Schizochytrium sp.) have been added in the diet of O. niloticus to substitute FM and FO in their feed. A significant improvement in ADCs of the crude protein, amino acids, lipid, and other fatty acids was observed in the farmed fish (Sarker et al., 2020b).

Integrated algae-aquaculture systems provide a suitable platform to develop ecofriendly, economical, and sustainable aquafeeds. Accordingly, different microalgae based FMs, the diet comprising 7.5% of whole and LEA has delivered essential nutrients with significant growth performance indicators (FCR 1.36 g/g, PER 1.84 g/g, and HSI 2.01%) in O. niloticus cultivated in a raceway pond (Ansari et al., 2020). Spotted wolf fish (Anarhichas minor) dominate the cold-water aquaculture. Its FM was substituted up to 15% of the N. oceanica that has resulted in high level of omega 3-fatty level in the muscle, liver, and whole body of all treatment sets, replicating the use of ~ 50% plant-based ingredients in the diets (Knutsen et al., 2019a). Moreover, Scenedesumus obliquus can be used as a substitute and valued feed constituent to partially replace FM to the existence of extraordinary protein content (above 50% of dry matter). Therefore, substituting 4% of FM has tremendously increased the body weight of the fish from 140 to 250 g after 12 weeks with rapid muscle growth and proximate arrangement of muscle suggesting the potential use of microalgae in aquaculture (Knutsen et al., 2019b).

Microalgae can be incorporated in FM with a particular percentage to progress the growth configurations, stress comebacks, liver functions, physiological events, and disease resistances of numerous fish varieties. For example, Lipid-extracted microlagae (LEA) meal mixed by soy protein (10% of the diet) to replace 25% of FM in the tested diet has shown good growth performance with growth indicators (FCR 1.19 g/g, PER 1.76 g/g, and HSI 2.00%) matched to the control diet in Perca flavescens (Jiang et al., 2019). According to Younis et al. (2018) the FM of O. niloticus is substituted by 20% G. arcuate that has significantly increased their body weight from 13.01 to 36.13 g after 12 weeks. Furthermore, Sarker et al. (2018) investigated that 33% LEA substitution of the FM has significant impact on the growth performance, feed utilization, and persistence comparable to control diet. After 12 weeks, the body weight increased from 1.98 to 28.06g with the growth indicators (FCR 1.26 g/g, and PER 2.12 g/g). Kiron et al. (2016) found that increasing the integration of Desmodesmus sp. as of 10% to 20% in FM has no adverse effects on the feed consumption and health of Salmo salar L. Similarly, Norambuena et al. (2015) found that Ulva ohnoi and Entomoneis spp. with inclusion levels of 2.5% and 5.0% could be added in FM of Salmo salar L that shows an enhanced feed efficacy (rich in n-3 LC-PUFA) related to the reference diets.

Digestible protein (DP) content is an important feed ingredient that is necessary for the development of new diet formulations for the acquaculture industry. The Protein degree of hydrolysis (DH) and predicted protein apparent digestibility coefficients (ADCs) of N. granulata algal meals can provided key parameters of its incorporation in the meals of L. vannamei (Tibbetts et al., 2017). In other report microalgae, concentrates have been used as larval feed of T. noae to determine its ingestion and digestion efficiency that was subjective to the type of microalgae and larval age (Southgate et al., 2017). The dietary potential of Schizochytrium as a meal supplement has been accessed in a feeding trial to investigate the survival, growth performance, digestive enzymes, and fatty acid configuration in the larvae of Litopenaeus vannamei. It was observed that addition of 4% Schizochytrium meal in microdiets of shrimps could progress their growth performance and other essential life activities (Wang et al., 2017). According to Kousoulaki et al. (2016), adding 5% whole biomass of Schizochtrum sp. in the extruded meal of Salmo salar L effectively replaced FO deprived of any adversarial effect on their growth performance, preservative ability of nutritional value. According to Kissinger et al. (2016) FM replaced by microalgae up to 80% showed no sizable effect on the growth performance or intestinal integrity of S. rivoliana with an improved body weight from 2.5 to 74.0 g after 9 weeks. Sørensen et al. (2016) scrutinized the whole cell microalgae Phaeodactylum tricornutum as an impending feed constituent for Salmo salar. A direct decline in apparent digestibility coefficients (ADC) was detected for protein, lipid, and DM to replace FM with P. tricornutum biomass from 0-12% in the feed. The algae biomass can substitute 6% of the FM devoid of any contrary impact on nutrient digestibility, growth and feed consumption of the fish (Sørensen et al., 2016).

The enhanced digestibility of crude protein and many essential amino acids found in Spirulina sp. recommend it as a good contender to be considered as an alternative protein source while Schizochytrium sp., contain maximum quantity of lipid and unsaturated fatty acids is a good candidate of FO substitute in tilapia feed. These microalgae species have been investigated for the apparent digestibility of macronutrients, amino acids and fatty acids in the Nile tilapia (Sarker et al., 2016). Similarly, the potential of Pavlova viridis as a PUFA source was assessed by comparing to Nannochloropsis sp. in the diets of Dicentrarchus labrax L. during 8-week feeding trial. It was observed that 50-100% microalgae could be added to replace FO in the diet of Dicentrarchus sp has no adverse effects on their growth performance and nutrient utilization (Haas et al., 2016). In another study the nutrient digestibility, growth performance, biometry, dressing out parameters, fillet muscle proximate and fatty acid composition of Dicentrarchus labrax L. have been investigated by replacing 20% PR and up to 36% FO with freeze-dried biomass of Isochrysis sp. has no adversative effects, feed intake or growth performance as compared to controls (Tibaldi et al., 2015). Another study has assessed the effects of diets comprising 0, 2.5, 5, 7.5 and 10% of Spirulina platensis on the hematological and serum biochemical factors of Oncorhynchus mykiss. It was found that replacing 10% diet with Arthrospira sp has significantly increased the red and white blood count, hemoglobin, total protein, and albumin levels in Oncorhynchus sp. (Yeganeh et al., 2015).


The current aquaculture industry is facing the problems related to the environmental safety and food security. Therefore, researchers have focused to resolve these problems to develop a sustainable aquaculture. The main problems in the customary aquaculture are water deterioration and antibiotics misuse that are not only responsible for resistance development but also polluting the water body (Han et al., 2019). The water deterioration generally occurs due to depletion of oxygen, detrimental algal bloom, and eutrophication of the water used in aquaculture. These problems possess serious threat for rearing the aquatic species as well as causes environmental pollution (Lu et al., 2019; Liu et al., 2014). One of the main causes of water deterioration is due to the excessive use of customary aquaculture feed comprised biomass contain protein and lipid that is not fully utilized by the aquatic animals. The remaining feed is changed to soluble nutrients by specific microorganisms ultimately causes eutrophication in water body (Han et al., 2019). Water deterioration also occurs due to the wastes secreted by the aquatic species that in the end can cause diseases in the aquatic animals ultimately their death (Lamb et al., 2017; Bhatnagar and Devi, 2013). To overcome these problems a biorefinery approach can be a cost-effective and sustainable paradigm in which microalgae cultivation and aquaculture, integrated for shared benefits (Shaalan et al., 2018). Figure 1 shows an integrated microlage-aquaculture system for sustainable aquaculture production.


The integrated system of using microalgae in aquaculture system is an emerging paradigm that can be adopted to develop an ecofriendly and sustainable aquaculture. Aquaculture industry still facing the problems of expansive aquafeeds while its conventional ingredients possess environmental issues. Considering these problems microalgae is the best candidate, which comprise essential nutrients, can replace FM and FO in aquafeeds of the aquatic species to produce cost-effective and high-quality nutritious food for the malnourished population of the developing counters. However, the diversity in microalgae strains, their nutrients composition, environmental factors, and formulation in aquafeeds needs further research. Moreover, an integrated and economically sustainable biorefinery approach can be implemented to cultivate microalgae as aquaculture feed whereas the aquaculture wastewater used as a nutrients source to produce useful biomass. Microalgae has significant environmental benefits of fixing carbon dioxide and wastewater remediation, thus reducing the pollution problem caused by aquaculture wastewater. Therefore, considering the environmental and economic facets, microalgae-assisted aquaculture needs to be developed that will open new avenues in aquaculture and environmental sustainability.


Microalgae have the potential to assimilate nitrate, nitrite, ammonia, phosphate, and organic carbon from aquaculture wastewater for their growth. Aquatic species generate these unwanted compounds in the wastewater body used for rearing. Therefore, microalgae are the best candidate to remove such nutrients and convert into useful biomass that can be used as feed constituent in aquafeeds. Thus, microalgae cultivation in wastewater has exclusive benefits in terms of bio-circular economy e.g., removing nutrients from the aquatic ecosystem and producing cost effective biomass for aquaculture industry. Subsequently biomass harvest, the treated water can be castoff for rearing aquatic organisms or other useful applications to develop a sustainable and ecofriendly environment (Yang et al., 2020).

A sustainable biorefinery approach was investigated in aquaculture wastewater of tilapia rearing tanks by growing Chlorella sorokiniana heterotrophically to utilize wastewater substrate for dual benefits (nutrients bioremediation and biomass generation). Microalgae has significantly removed phosphate, ammonia, nitrate, and COD (chemical oxygen demand) as 73.35, 75.56, 84.51, and 71.88% from the aquaculture wastewater with biomass productivity comprised proteins, lipids, and carbohydrates as 141.57, 150.19 and 172.91 mg/L/day (Guldhe et al., 2017). Similarly, Scenedesmus obliquus, Chlorella sorokiniana and Ankistrodesmus falcatus were cultivated in aquaculture wastewater (AWW) to investigate the biorefinary model to produce biomass with subsequent nutrient removal. A. falcatus generated biomass of 198.46 mg L−1d−1 with added sodium nitrate (400 mg L−1) while C. sorokiniana produced biomass of 157.04 mg L−1d−1 with supplemented sodium nitrate (600 mg L−1) in AWW as compared to the BG11 medium. Microalgae grown in AWW showed significant removal of ammonia, nitrate, phosphate, and COD in the range of 86.45-98.21, 75.76-80.85, 98.52-100 and 42-69% respectively (Ansari et al., 2017).

The wastewater of the recirculating aquaculture system (RAS) was used as nutrient medium to co-cultivate two different species of microalgae (C. vulgaris and T. obliquus). Both strains grow vigorously than their monoculture with average removal efficiencies of nitrate (98.73±0.06) and phosphate (99.46±0.04%), respectively (Tejido-Nuñez et al., 2020). Numerous strains of microalgae have been grown in aquaculture wastewater for nutrients bioremediation and biomass generation for an ecofriendly and sustainable aquaculture (Peng et al., 2020; Nasir et al., 2019; Gupta et al., 2016). Similarly, consortia of microalgae and associated water-borne bacteria through their extracellular enzymatic activities can potentially remediate the compact wastes of aquatic species. In such a symbiotic relationship, microalgae can effectively assimilate the nutrients from the AWW refining the self-purification capability of aquaculture system by producing high value biomass (Addy et al., 2017; Fang et al., 2017).


Aquaculture is a fast-growing sector playing an important role in providing high quality seafood mainly depends on FM and FO in the aquafeeds. Microalgae contain valued source of the essential nutrients required for high quality aquafeeds, comprising omega-3 fatty acids, EPA and DHA, essential amino acids, pigments, and antioxidants. Microalgae is the best source to replace FM and FO in aquafeeds due to their important role in the enhanced growth performance, physiological movement, and nutritional status of the aquatic species. Moreover, the integrated microalgae-aquaculture system provides a sustainable biorefinery approach e.g., removing the wastes of aquatic organisms and converting into cost-effective biomass. Therefore, microalgae-assisted aquaculture is necessary to develop a sustainable circular bioeconomy.


The author highly acknowledge the facilities provided by the Department of Bioengineering, King Fahd University of Petroleum and Minerals (KFUPM), Dhahran, Kingdom of Saudi Arabia for the conduction of this study.

Statement of conflict of interest

The author has declared no conflict of interest.


Acquah, C., Tibbetts, S.M., Pan, S., and Udenigwe, C., 2020. Nutritional quality and bioactive properties of proteins and peptides from microalgae. In: Handbook of microalgae-based processes and products (eds. E. Jacob-Lopes, M.M. Maroneze, M.I. Queiroz, and L.Q. Zepka). Elsevier/Academic Press, London (UK). 1: 493-531.

Addy, M.M., Kabir, F., Zhang. R., Lu, Q., Deng, X., Current, D., Griffith, R., Ma, Y., Zhou, W., Chen, P., and Ruan, R. 2017. Co-cultivation of microalgae in aquaponic systems. Bioresour. Technol., 245(Pt A): 27-34.

Alfnes, F., Guttormsen, A.G., Steine, G., and Kolstad, K. 2006. Consumers’ willingness to pay for the color of salmon: a choice experiment with real economic incentives. Am. J. agric. Econ., 88: 1050-1061.

Ambati, R.R., Gogisetty, D., Aswathanarayana, R.G., Ravi, S., Bikkina, P.N., Bo, L., and Yuepeng, S. 2019. Industrial potential of carotenoid pigments from microalgae: Current trends and future prospects. Crit. Rev. Fd. Sci. Nutr., 59: 1880-1902.

Ambati, R.R., Moi, P.S., Ravi, S., and Aswathanarayana, R.G., 2014. Astaxanthin: Sources, extraction, stability, biological activities and its commercial applications. A review. Mar. Drugs, 12: 128-152.

Amna, K.S., Hwang, Y.J., and Park, J.K., 2018. Potent biomedical applications of isolated polysaccharides from marine microalgae Tetraselmis species. Bioproc. Biosyst. Eng., 41: 1611-1620.

Ansari, F.A., and Gupta, S.K., 2019. Microalgae: A biorefinary approach to the treatment of aquaculture wastewater. In: Application of microalgae in wastewater treatment. Springer Nature, Switzerland AG 2019 (eds. S.K. Gupta and F. Bux). pp. 69.

Ansari, F.A., Guldhe, A., Gupta. S.K., Rawat, I., and Bux, F., 2021. Improving the feasibility of aquaculture feed by using microalgae. Environ. Sci. Pollut. Res. Int., 28: 43234-43257.

Ansari, F.A., Nasr, M., Guldhe, A., Gupta, S.K., Rawat, I., and Bux, F., 2020. Techno-economic feasibility of algal aquaculture via fish and biodiesel production pathways: A commercial-scale application. Sci. Total Environ., 704: 135259.

Ansari, F.A., Guldhe, A., Gupta, S.K., Rawat, I., and Bux, F., 2021. Improving the feasibility of aquaculture feed by using microalgae. Environ. Sci. Pollut. Res. Int., 28: 43234-43257.

Ansari, F.A., Singh, P., Guldhe, A., and Bux, F., 2017. Microalgal cultivation using aquaculture wastewater: Integrated biomass generation and nutrient remediation. Algal Res., 21: 169-177.

Arney, B., Liu, W., Forster, I.P., McKinley, R.S., and Pearce, C.M., 2015. Feasibility of dietary substitution of live microalgae with spray-dried Schizochytrium sp. or Spirulina in the hatchery culture of juveniles of the Pacific geoduck clam (Panopea generosa). Aquaculture, 444: 117-133.

Bélanger, A., Sarker, P.K., Bureau, D.P., Chouinard, Y., and Vandenberg, G.W., 2021. Apparent digestibility of macronutrients and fatty acids from microalgae (Schizochytrium sp.) fed to rainbow trout (Oncorhynchus mykiss): A potential candidate for fish oil substitution. Animals, 11: 456.

Bellou, S., Baeshen, M.N., Elazzazy, A.M., Aggeli, D., Sayegh, F., and Aggelis, G., 2014. Microalgal lipids biochemistry and biotechnological perspectives. Biotechnol. Adv., 32: 1476-1493.

Bernaerts, T.M., Gheysen, L., Kyomugasho, C., Kermani, Z.J., Vandionant, S., Foubert, I., Hendrickx, M.E., and Van Loey, A.M., 2018. Comparison of microalgal biomasses as functional food ingredients: Focus on the composition of cell wall related polysaccharides. Algal Res., 32: 150-161.

Bernaerts, T.M., Gheysen, L., Foubert, I., Hendrickx, M.E., and Van Loey, A.M., 2019. The potential of microalgae and their biopolymers as structuring ingredients in food: A review. Biotechnol. Adv., 37: 107419.

Besen, K.P., Melim, E.W.H., da Cunha, L., Favaretto, E.D., Moreira, M., and Fabregat, T.E.H.P., 2019. Lutein as a natural carotenoid source: Effect on growth, survival and skin pigmentation of goldfish juveniles (Carassius auratus). Aquacult. Res., 50: 2200-2206.

Bhatnagar, A., and Devi, P., 2013. Water quality guidelines for the management of pond fish culture. Int. J. environ. Sci., 3: 1980.

Blomqvist, J., Pickova, J., Tilami, S.K., Sampels, S., Mikkelsen, N., Brandenburg, J., Sandgren, M., and Passoth, V., 2018. Oleaginous yeast as a component in fish feed. Sci. Rep., 8: 1-8.

Bongiorno, T., Foglio, L., Proietti, L., Vasconi, M., Lopez, A., Pizzera, A., Carminati, D., Tava, A., Vizcaíno, A.J., Alarcón, F.J., Ficara, E., and Parati, K., 2020. Microalgae from biorefinery as potential protein source for siberian sturgeon (A. baerii) aquafeed. Sustainability, 12: 8779.

Bou, M., Berge, G.M., Baeverfjord, G., Sigholt, T., Ostbye, T.K., Romarheim, O.H., Hatlen. B., Leeuwis. R., Venegas, C., and Ruyter, B., 2017. Requirements of n-3 very long-chain PUFA in Atlantic salmon (Salmo salar L): Effects of different dietary levels of EPA and DHA on fish performance and tissue composition and integrity. Br. J. Nutr., 117: 30-47.

Butler, T., McDougall, G., Campbell, R., Stanley, M., and Day, J., 2018. Media screening for obtaining Haematococcus pluvialis red motile macrozooids rich in astaxanthin and fatty acids. Biology, 7: 2.

Chen, F., Xiao, Y., Wu, X., Zhong, Y., Lu, Q., and Zhou, W., 2020. Replacement of feed by fresh microalgae as a novel technology to alleviate water deterioration in aquaculture. RSC Adv., 10: 20794.

Chrapusta, E., Kaminski, A., Duchnik, K., Bober, B., Adamski, M., and Bialczyk, L., 2017. Mycosporine-like amino acids: Potential health and beauty ingredients. Mar. Drugs, 15: 326.

Chronakis, I.S., and Madsen, M., 2011. Algal proteins. Handbook of food proteins. In: Woodhead publishing series in food sciences, technology and nutrition (eds. G.O. Phillips and P.A. Williams). pp. 353-394.

Colombo, S.M., 2020. Chapter 3: Physiological considerations in shifting carnivorous fishes to plant-based diets. In: Fish physiology. 38th Edition (eds. T.J. Benfey, A.P. Farrell and C.J. Brauner). Elsevier.

Conceicao, L.E.C., Yufera, M., Makridis, P., Morais, S., and Dinis, M.T., 2010. Live feeds for early stages of fish rearing. Aquacult. Res., 41: 613-640.

Costa, D.P., and Miranda-Filho, K.C., 2019. The use of carotenoid pigments as food additives for aquatic organisms and their functional roles. Rev. Aquacult., 12: 1567-1578.

Cottrell, R.S., Blanchard, J.L. Halpern, B.S., Metian, M. and Froehlich, H.E., 2020. Global adoption of novel aquaculture feeds could substantially reduce forage fish demand by 2030. Nat. Fd., 1: 301-308.

Daneshvar, E., Zarrinmehr, M.J., and Hashtjin, A.M., 2018. Versatile applications of freshwater and marine water microalgae in dairy wastewater treatment, lipid extraction and tetracycline biosorption. Bioresour. Technol., 268: 523-530.

Del Mondo, A., Smerilli, A., Sané, E., Sansone, C., and Brunet, C., 2020. Challenging microalgal vitamins for human health. Microb. Cell Fact., 19: 201.

Draaisma, R.B., Wijffels, R.H., Slegers, P.M.E., Brentner, L.B., Roy, A., and Barbosa, M.J., 2013. Food commodities from microalgae. Curr. Opin. Biotechnol., 24: 169-177.

El-Sayed, A.F.M., 1994. Evaluation of soybean meal, spirulina meal and chicken offal meal as protein sources for silver seabream (Rhabdosargus sarba) fingerlings. Aquaculture, 127: 169-176.

Fang, Y., Hu, Z., Zou, Y., Fan, J., Wang, Q., and Zhu, Z., 2017. Increasing economic and environmental benefits of media-based aquaponics through optimizing aeration pattern. J. Clean. Prod., 162: 1111-1117.

FAO, 2016. The state of world fisheries and aquaculture 2016. Contributing to food security and nutrition for all. (Report No. ISBN 978-92-5-109185-2). Report by United Nations (UN).

FAO, 2018. The state of world fisheries and aquaculture 2018. Meeting the sustainable development goals. (Report No. ISBN 978-92-5-130562-1). Report by United Nations (UN).

FAO, 2020. The state of world fisheries and aquaculture 2020. Sustainability in action. Rome.

Fox, J.M., and Zimba, P.V., 2018. Minerals and trace elements in microalgae. In: Microalgae in health and disease prevention. Academic Press. pp. 177-193.

Froehlich, H., Jacobsen, N.S., Essington, T.E., Clavelle, T., and Halpern, B.S., 2018. Avoiding the ecological limits of forage fish for fed aquaculture. Nat. Sustain., 1: 298-303.

Fry, J.P., Love, D.C., MacDonald, G.K., West, P.C., Engstrom, P.M., Nachman, K.E., and Lawrence, R.S., 2016. Environmental health impacts of feeding crops to farmed fish. Environ. Int., 91: 201-214.

Galal, A.A.A., Reda, R.M., and Abdel-Rahman, M.A., 2018. Influences of Chlorella vulgaris dietary supplementation on growth performance, hematology, immune response and disease resistance in Oreochromis niloticus exposed to sub-lethal concentrations of penoxsulam herbicide. Fish Shellfish Immun., 77: 445-456.

Garcıa-chavarrıa, M., and Lara-Flores, M., 2013. The use of carotenoid in aquaculture. J. Fish. Hydrobiol., 8: 38-49.

Guldhe, A., Ansari, F.A., Singh, P., and Bux, F., 2017. Heterotrophic cultivation of microalgae using aquaculture wastewater: A biorefinery concept for biomass production and nutrient remediation. Ecol. Eng., 99: 47-53.

Gupta, S.K., Ansari, F.A., Shriwastav, A., Sahoo, N.K., Rawat, I., and Bux, F., 2016. Dual role of Chlorella sorokiniana and Scenedesmus obliquus for comprehensive wastewater treatment and biomass production for bio-fuels. J. Clean Prod., 115: 255-264.

Güroy, B., Şahin, İ., Mantoğlu, S., and Kayalı, S., 2012. Spirulina as a natural carotenoid source on growth, pigmentation and reproductive performance of yellow tail cichlid Pseudotropheus aceiAquacult. Int., 20: 869-878.

Haas, S., Bauer, J.L., Adakli, A., Meyer, S., Lippemeier, S., Schwarz, K., and Schulz, C., 2016. Marine microalgae Pavlova viridis and Nannochloropsis sp. as n-3 PUFA source in diets for juvenile European sea bass (Dicentrarchus labrax L.). J. appl. Phycol., 28: 1011-1021.

Han, P., Lu. Q., Fan, L., and Zhou, W., 2019. A review on the use of microalgae for sustainable aquaculture. Appl. Sci., 9: 2377.

Hardwood, J.L., 2019. Algae: Critical sources of very long-chain polyunsaturated fatty acids. Biomolecules, 9: 708.

Hart, B., Schurr, R., Narendranath, N., Kuehnle, A., and Colombo, S.M., 2021. Digestibility of Schizochytrium sp. whole cell biomass by Atlantic salmon (Salmo salar). Aquaculture, 533: 736156.

He, Y., Lin, G., Rao, X., Chen, L., Jian, H., Wang, M., Guo, Z., and Chen, B., 2018. Microalga Isochrysis galbana in feed for Trachinotus ovatus: effect on growth performance and fatty acid composition of fish fillet and liver. Aquacult. Int., 26: 1261-1280.

Hemaiswarya, S., Raja, R., Kumar, R.R., Ganesan, V., and Anbazhagan, C., 2011. Microalgae: A sustainable feed source for aquaculture. World J. Microbiol. Biotechnol., 27: 1737-1746.

Hodar, A., Vasava, R., Mahavadiya, D., Joshi, N., 2020. Fish meal and fish oil replacement for aqua feed formulation by using alternative sources: A review. J. exp. Zool. India, 23: 13-21.

Hua, K., Cobcroft, M.J.M., Cole, A., Condon, K., Jerry, D.R., Mangott, A., Praeger, C., Vucko, M.J., Zeng, C., Zenger, K., and Strugnell, J.M., 2019. The future of aquatic protein: implications for protein sources in aquaculture diets. One Earth, 1: 316-329.

Ibrahim, M.A., Al-Thukair, Shaikh, A.R., Farooq, W., and Ahmad, I., 2020. Isolation of indigenous microalgae: Nitrogen/phosphorousremoval and biofuel production. Biofuels, 11: 269-276.

Jiang, M., Zhao, H.H., Zai, S.W., Shepherd, B., Wen, H., and Deng, D.F., 2019. A defatted microalgae meal (Haematococcus pluvialis) as a partial protein source to replace fishmeal for feeding juvenile yellow perch Perca flavescens. J. appl. Phycol., 31: 1197-1205.

Kiran, B.R., and Mohan, V.S., 2021. Microalgal cell biofactory-therapeutic, nutraceutical and functional food applications. Plants, 10: 836.

Kiron, V., Sørensen, M., Huntley, M., Vasanth, G.K., Gong, Y., Dahle, D., Palihawadana, A.M., and Palihawadana. 2016. Defatted biomass of the microalga, Desmodesmus sp., can replace fishmeal in the feeds for atlantic salmon. Front. Mar. Sci., 3: 67.

Kissinger, K., García-Ortega, A., and Trushenski, J., 2016. Partial fish meal replacement by soy protein concentrate, squid and algal meals in low fish-oil diets containing Schizochytrium limacinum for longfin yellowtail Seriola rivoliana. Aquaculture, 452: 37-44.

Knutsen, H.R., Johnsen, I.H., Keizer, S., Sørensen, M., Roques, J.A.C., Hedén, I., Sundell, K., and Hagen, Ø., 2019a. Fish welfare, fast muscle cellularity, fatty acid and body-composition of juvenile spotted wolffish (Anarhichas minor) fed a combination of plant proteins and microalgae (Nannochloropsis oceanica). Aquaculture, 506: 212-223.

Knutsen, H.R., Ottesen, O.H., Palihawadana, A.M., Sandaa, W., Sørensen, M., and Hagen, Ø., 2019b. Muscle growth and changes in chemical composition of spotted wolffish juveniles (Anarhichas minor) fed diets with and without microalgae (Scenedesmus obliquus). Aquacult. Rep., 13: 100175.

Kousoulaki, K., Mørkøre, T., Nengas, I., Berge, R.K., and Sweetman, J., 2016. Microalgae and organic minerals enhance lipid retention efficiency and fillet quality in Atlantic salmon (Salmo salar L.). Aquaculture, 451: 47-57.

Lamb, J.B., van de Water, J.A., Bourne, D.G., Altier, C., Hein, M.Y., Fiorenza, E.A., Abu, N., Jompa, J., and Harvell, C.D., 2017. Seagrass ecosystems reduce exposure to bacterial pathogens of humans, fishes, and invertebrates. Science, 355: 731-733.

Laye, S., Nadjar, A., Joffre, C., and Bazinet, R.P., 2018. Anti-inflammatory effects of omega-3 fatty acids in the brain: Physiological mechanisms and relevance to pharmacology. Pharmacol. Rev., 70: 12-38.

Lehnert, S.J., Christensen, K.A., Vandersteen, W.E., Sakhrani, D., Pitcher, T.E., Heath, J.W., Koop, B.F., Heath, D.D., and Devlin, R.H., 2019. Carotenoid pigmentation in salmon: variation in expression at BCO2-l locus controls a key fitness trait affecting red coloration. Proc. R. Soc. B., 286: 20191588.

Li, S., Wang, B., Liu, L., Song, Y., Lv, C., Zhu, X., Luo, Y., Cheng, C.H.K., Chen, H., Yang, X., and Li, T., 2021. Enhanced growth performance physiological and biochemical indexes of Trachinotus ovatus fed with marine microalgae Aurantiochytrium sp. Rich in n-3 polyunsaturated fatty acids. Front. Mar. Sci., 7: 609837.

Liu, X., Xu. H., Wang, X., Wu, Z., and Bao, X., 2014. An ecological engineering pond aquaculture recirculating system for effluent purification and water quality control. Clean Soil Air Water, 42: 221-228.

Liu, W.Y., Fang, X.W., Li, G.M., and Gu, R.Z., 2020. In vitro antioxidant and angiotensin 1-converting enzyme inhibitory properties of peptides derived from corn gluten meal. Eur. Fd. Res. Technol., 446: 2017-2027.

Lu, Q., Han, P., Xiao, Y., Liu, T., Chen, F., Leng, L., Liu, H., and Zhou, J., 2019. The novel approach of using microbial system for sustainable development of aquaponics. J. Clean Prod., 217: 573-575.

Lyons, P.P., Turnbull, J.F., Dawson, K.A., and Crumlish, M., 2017. Effects of low-level dietary microalgae supplementation on the distal intestinal microbiome of farmed rainbow trout Oncorhynchus mykiss (Walbaum). Aquacult. Res., 48: 2438-2452.

Ma, Y., Wang, A., Yu, C., Yin, Y., and Zhou, G., 2014. Evaluation of the potential of 9 Nannochloropsis strains for biodiesel production. Bioresour. Technol., 167: 503-509.

Maliwat, G.C., Velasquez, S., Robil, J.L., Chan, M., Traifalgar, R.F., and Tayamen, M. and Ragaza, J.A., 2017. Growth and immune response of giant freshwater prawn Macrobrachium rosenbergii (De Man) postlarvae fed diets containing Chlorella vulgaris (Beijerinck). Aquacult. Res., 48: 1666-1676.

Maliwat, G.C.F., Velasquez, S.F., Buluran, S.M.D., Tayamen, M.M., and Ragaza, J.A., 2021. Growth and immune response of pond-reared giant freshwater prawn Macrobrachium rosenbergii post larvae fed diets containing Chlorella vulgaris. Aquacult. Fish., 6: 465-470.

Madeira, M.S., Cardoso, C., Lopes, P.A., Coelho, D., Afonso, C., Bandarra, N.M., and Prates, J.A., 2017. Microalgae as feed ingredients for livestock production and meat quality: A review. Livest. Sci., 205: 111-121.

Marudhupandi, T., and Inbakandan, D., 2015. Polysaccharides in aquatic disease management. Fish. Aquacult. J., 6: 3.

Moha-Leon, J.D., Perez-Legaspi, I.A., Hernandez-Vergara, M.P., Perez-Rostr, C.I., and Clark-Tapia, R., 2018. Study of the effects of photoperiod and salinity in the Alvarado strain of the Brachionus plicatilis species complex (Rotifera: Monogononta). Annls Limnol. Int. J. Lim., 51: 335-342.

Mohan, K., Ravichandran, S., Muralisankar, T., Uthayakumar, V., Chandirasekar, R., Seedevi, P., Abirami, R.G., and Rajan, D.K., 2019. Application of marine-derived polysaccharides as immunostimulants in aquaculture: A review of current knowledge and further perspectives. Fish Shellfish Immunol., 86: 1177-1193.

Nasir, N.M., Yunos, F.H.M., Jusoh, H.H.W., Mohammad, A., Lam, S.S., and Jusoh, A., 2019. Subtopic: advances in water and wastewater treatment harvesting of Chlorella sp. microalgae using Aspergillus niger as bioflocculant for aquaculture wastewater treatment. J. environ. Manag., 249: 109373.

Norambuena, F., Hermon, K., Skrzypczyk, V., Emery, J.A., Sharon, Y., Beard, A., and Turchini, G.M., 2015. Algae in fish feed: Performances and fatty acid metabolism in juvenile Atlantic salmon. PLoS One, 10: e0124042.

Novoveská, L., Ross, M.E., Stanley, M.S., Pradelles, R., Wasiolek, V., and Sassi, J.F., 2019. Microalgal carotenoids: A review of production, current markets, regulations, and future direction. Mar. Drugs, 17: 640.

Oliver, L., Dietrich, T., Marañón, I., Villarán, M.C., and Barrio, R.J., 2020. Producing omega-3 polyunsaturated fatty acids: A review of sustainable sources and future trends for the EPA and DHA market. Resources9: 48.

Paerl, H.W., and Otten, T.G., 2012. Harmful cyanobacterial blooms: Causes, consequences, and controls. Microb. Ecol., 65: 995-1010.

Pascon, G., Messina, M., Petit, L., Valente, L.M.P., Oliveira, B., Przybyla, C., Dutto, G., and Tulli, F., 2021. Potential application and beneficial effects of a marine microalgal biomass produced in a high-rate algal pond (HRAP) in diets of European sea bass, Dicentrarchus labraxEnviron. Sci. Pollut. Res., 28: 62185-62199.

Patras, D., Moraru, C.V., and Socaciu, C., 2019. Bioactive ingredients from microalgae: Food and feed applications. Buasvmcn-Fst, 76: 1-9.

Peng, Y.Y., Gao, F., Yang, H.L., Li, C., Lu, M.M., and Yang, Z.Y., 2020. Simultaneous removal of nutrient and sulfonamides from marine aquaculture wastewater by concentrated and attached cultivation of Chlorella vulgaris in an algal biofilm membrane photobioreactor (BF-MPBR). Sci. Total Environ., 725: 138524.

Perez-Legaspi, I., Guzman-Ferman, B., Moha-Leon, J.D., Ortega- Clemente, L.A., Valadez- and Rocha, V., 2018. Effects of the biochemical composition of three microalgae on the life history of the rotifer Brachionus plicatilis (Alvarado strain): An assessment. Annls Limnol. Int. J. Lim., 54: 2-8.

Postma, P.R., Miron, T.L., Olivieri, G., Barbosa, M.J., Wijffels, R.H.; and Eppink, M.H.M., 2015. Mild disintegration of the green microalgae Chlorella vulgaris using bead milling. Bioresour. Technol., 184: 297-304.

Prybylski, N., Toucheteau, C., El Alaoui, H., Bridiau, N., and Maugard, T., Abdelkafi, S., Fendri, I., Delattre, C., Dubessay, Pierre, G. and Michaud P., 2020. Bioactive polysaccharides from microalgaeHandbook of microalgae-based processes and products. Elsevier. pp. 533-571.

Radhakrishnan, S., Belal, I.E.H., Seenivasan, C., Muralisankar, T., and Bhavan, P.S., 2016. Impact of fishmeal replacement with Arthrospira platensis on growth performance, body composition and digestive enzyme activities of the freshwater prawn, Macrobrachium rosenbergii. Aquacult. Rep., 3: 35-44.

Raposo, M.F., de Morais, R.M., and Bernardo de Morais, A.M., 2013. Bioactivity and applications of sulphated polysaccharides from marine microalgae. Mar. Drugs, 11: 233-252.

Remize, M., Brunel, Y., Silva, J.L., Berthon, J.Y., and Filaire, E., 2021. Microalgae n-3 PUFAs production and use in food and feed industries. Mar. Drugs, 19: 113.

Ryckebosch, E., Bruneel, C., Termote-Verhalle, R., Goiris, K., Muylaert, K., and Foubert, I., 2014. Nutritional evaluation of microalgae oils rich in omega-3 long chain polyunsaturated fatty acids as an alternative for fish oil. Fd. Chem., 160: 393-400.

Saini, R.K., and Keum, Y.S., 2018. Omega-3 and omega-6 polyunsaturated fatty acids: dietary sources, metabolism, and significance. A review. Life Sci., 203: 255-267.

Samuelsen, T.A., Oterhals, A., and Kousoulaki, K., 2018. High lipid microalgae (Schizchytrium sp.) inclusion as a sustainable source of 3-n long-chain PUFA in fish feed-effects on the extrusion process and physical pellet quality. Anim. Feed Sci. Technol., 236: 14-28.

Sarker, P.K., Kapuscinski, A.R., Bae, A.Y., Donaldson, E., Sitek, A.J., Fitzgerald, D.S., and Edelson, O.F., 2018. Towards sustainable aquafeeds: Evaluating substitution of fishmeal with lipid-extracted microalgal co-product (Nannochloropsis oculata) in diets of juvenile Nile tilapia (Oreochromis niloticus). PLoS One, 13: e0201315.

Sarker, P.K., Kapuscinski, A.R., McKuin, B., Fitzgerald, D.S., Nash, H.M., and Greenwood, C., 2020a. Microalgae-blend tilapia feed eliminates fishmeal and fish oil, improves growth, and is cost viable. Sci. Rep., 10: 19328.

Sarker, P.K., Kapuscinski, A.R., Vandenberg, G.M., Proulx, E., and Sitek, A.J., 2020b. Towards sustainable and ocean-friendly aquafeeds: Evaluating a fish-free feed for rainbow trout (Oncorhynchus mykiss) using three marine microalgae species. Elem. Sci. Anth., 8: 5.

Sarker, P.K., Gamble, M.M., Kelson, S., and Kapuscinski, A.R., 2016. Nile tilapia (Oncorhynchus niloticus) show high digestibility of lipid and fatty acids from marine Schizochytrium sp. and of protein and essential amino acids from freshwater Spirulina sp. feed ingredients. Aquacult. Nutr., 22: 109-119.

Shaalan, M., El-Mahdy, M., Saleh, M., and El-Matbouli, M., 2018. Aquaculture in Egypt: Insights on the current trends and future perspectives for sustainable development. Rev. Fish. Sci. Aquacult., 26: 99-110.

Shah, M.R., Lutzu, G.A., Alam, A., Sarker, P., Kabir Chowdhury, M.A., and Parsaeimehr, A., Liang, Y. and Daroch, M., 2017. Microalgae in aquafeeds for a sustainable aquaculture industry. J. Appl. Phycol., 30: 197-213.

Shah, M.R., Liang, Y., Cheng, J.J., and Daroch, M., 2016. Astaxanthin producing green microalga Haematococcus pluvialis from single cell to high-value commercial products. Front. Pl. Sci., 7: 531.

Silva, B., Wendt, E., Castro, J., de Oliveira, A., Carrim, A., Vieira, J., Sassi, R., Sassi, C., da Silva, A., Barboza, G., and Filho, N., 2015. Analysis of some chemical elements in marine microalgae for biodiesel production and other uses. Algal Res., 9: 312-321.

Siscovick, D.S., Barringer, T.A., Fretts, A.M., Wu, J.H.Y., Lichtenstein, A.H., Costello, R.B., Kris-Etherton, P.M., Jacobson, T.A., Engler, M.B., and Alger, H.M., Appel, L.J. and Mozaffarian, D., 2017. Omega-3 polyunsaturated fatty acid (fish oil) supplementation and prevention of clinical cardiovascular disease: A science advisory from the American heart association. Circulaiton, 135: e867-e884.

Sørensen, M., Morken, T., Kosanovic, M., and Overland, M., 2011. Pea and wheat starch possess different processing characteristics and effect physical quality and viscosity of extruded feed for Atlantic salmon. Aquacult. Nutr., 17: e326-e336.

Sørensen, M., Berge, G.M., Reitan, K.I., and Ruyter, B., 2016. Microalga Phaeodactylum tricornutum in feed for the Atlantic salmon (Salmo salar) effect on nutrient digestibility, growth and utilization of feed. Aquaculture, 460: 116-123.

Southgate, P.C., Braley, R.D., and Militz, T.A., 2017. Ingestion and digestion of micro-algae concentrates by veliger larvae of the giant clam. Tridacna Noae Aquacult., 473: 443-448.

Souza, F.P.D., Lima, E.C.S.D., Urrea-Rojas, A.M., Suphoronski, S.A., Facimoto, C.T., Bezerra and Ju´nior, J.D.S., Ju´nior, J.S.B., Oliveira, T.E.S., Pereira, U.P., Santis, G.W., Oliveira, C.A.L. and Lopera-Barrero, N.M., 2020. Effects of dietary supplementation with a microalga (Schizochytrium sp.) on the hemato-immunological, and intestinal histological parameters and gut microbiota of Nile tilapia in net cages. PLoS One, 15: e0226977.

Sprague, M., Dick, J.R., and Tocher, D.R., 2016. Impact of sustainable feeds on omega-3 longchain fatty acid levels in farmed Atlantic salmon, 2006-2015. Sci. Rep., 6: 1-9.

Tarento, T.D., McClure, D.D., Vasiljevski, E., Schindeler, A., Dehghani, F., and Kavanagh, J.M., 2018. Microalgae as a source of vitamin K1. Algal Res., 36: 77-87.

Tavakoli, S., Regenstein, J.M., Daneshvar, E., Bhatnagar, A., Luo, Y., and Hong, H., 2021. Recent advances in the application of microalgae and its derivatives for preservation, quality improvement, and shelf-life extension of seafood. Cret. Rev. Fd. Sci. Nutr., 1: 14.

Tejido-Nuñez, Y., Aymerich, E., Sancho, L., and Refardt, D., 2020. Cocultivation of microalgae in aquaculture water: Interactions, growth and nutrient removal efficiency at laboratory-and pilot-scale. Algal Res., 49: 101940.

Tibaldi, E., Zittelli, G.C., Parisi, G., Bruno, M., Giorgi, G., Tulli, F., Venturini, S., Tredici, M.R., and Poli, B.M., 2015. Growth performance and quality traits of European sea bass (D. labrax) fed diets including increasing levels of freeze-dried Isochrysis sp. (T. ISO) biomass as a source of protein and n-3 long chain PUFA in partial substitution of fish derivatives. Aquaculture, 440: 60-80.

Tibbetts, S., Milley, J., and Lall, S., 2015. Chemical composition and nutritional properties of freshwater and marine microalgal biomass cultured in photobioreactors. J. Appl. Phycol., 27: 1109-1119.

Tibbetts, S.M., 2018. The potential for next-generation, microalgae-based feed ingredients for salmonid aquaculture in context of the blue revolution. In: Microalgal Biotechnol., pp. 151-175.

Tibbetts, S.M., Yasumaru, F., and Lemos, D., 2017. In vitro prediction of digestible protein content of marine microalgae (Nannochloropsis granulata) meals for Pacific white shrimp (Litopenaeus vannamei) and rainbow trout (Oncorhynchus mykiss). Algal Res., 21: 76-80.

Turchini, G.M., Trushenski, J.T., and Glencross, B.D., 2019. Toughts for the future of aquaculture nutrition: Realigning perspectives to refect contemporary issues related to judicious use of marine resources in aquafeeds. N. Am. J. Aquacult., 81: 13-39.

Varelas, V., 2019. Food wastes as a potential new source for edible insect mass production for food and feed: A review. Fermentation, 5:81.

Wang, Y., Li, M., Filer, K., Xue, Y., Ai, Q., and Mai, K., 2017. Evaluation of Schizochytrium meal in microdiets of Pacific white shrimp (Litopenaeus vannamei) larvae. Aquacult. Res., 48: 2328-2336.

Woortman, D.V., Fuchs, T., Striegel, L., Fuchs, M., Weber, N., Brück, T.B., and Rychlik, M., 2020. Microalgae a superior source of folates: Quantification of folates in halophile microalgae by stable isotope dilution assay. Front. Bioeng. Biotechnol., 7: 481.

Yang, L., Wang, R., Lu, Q., and Liu, H., 2020. Algaquaculture integrating algae-culture with aquaculture for sustainable development. J. Clean Prod., 244: 118765.

Yang, Y., Park, J., You, S.G., and Hong, S., 2019. Immuno-stimulatory effects of sulfated polysaccharides isolated from Codium fragile in olive flounder, Paralichthys olivaceus. Fish Shellfish Immunol., 87: 609-614.

Yarnold, J., Karan, H., and Oey, M., 2019. Microalgal aquafeeds as part of a circular bioeconomy. Trends Pl. Sci., 24: 959-970.

Yeganeh, S., Teimouri, M., and Amirkolaie, A.K., 2015. Dietary effects of Spirulina platensis on hematological and serum biochemical parameters of rainbow trout (Oncorhynchus mykiss). Res. Vet. Sci., 101: 84-88.

Viegas, C., Gouveia, L., and Gonçalves, 2021. Aquaculture wastewater treatment trough microalgal.Biomass potential applications on animal feed, agricultural, and energy. J. environ. Manage., 286:112187.

Younis, E.S.M., Al-Quffail, A.S., Al-Asgah, N.A., Abdel-Warith, A.W.A., and Al-Hafedh, Y.S., 2018. Effect of dietary fishmeal replacement by red algae, Gracilaria arcuata, on growth performance and body composition of Nile tilapia Oreochromis niloticus. Saudi J. biol. Sci., 25: 198-203.

Yu, R., Cao, H., Huang, Y., Peng, M., Kajbaf, K., Kumar, V., Tao, Z., Yang, G., Wen, C., 2020. The effects of partial replacement of fishmeal protein by hydrolysed feather meal protein in the diet with high inclusion of plant protein on growth performance, fillet quality and physiological parameters of Pengze crucian carp (Carassius auratus var. Pengze). Aquacult. Res., 51: 636-647.

Zahran, E., Awadin, W., Risha, E., Khaled, A.A., and Wang, T., 2018. Dietary supplementation of Chlorella vulgaris ameliorates chronic sodium arsenite toxicity in Nile tilapia Oreochromis niloticus as revealed by histopathological, biochemical and immune gene expression analysis. Fish. Sci., 85: 199-215.

To share on other social networks, click on any share button. What are these?

Pakistan Journal of Zoology


Vol. 54, Iss. 6, Pages 2501-3000


Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits

Subscribe Unsubscribe