Population Analysis Based on Mito-nuclear Sequences: Implication of Dugesia japonica Decline in Taihang Mountains, China
He-Cai Zhang, Tian-Ge Hu, Chang-Ying Shi, Guang-Wen Chen* and De-Zeng Liu
Distribution map of D. japonica populations studied in Taihang Mountains. The red, blue, yellow and green areas represent Beijing Municipality, Hebei, Shanxi and Henan provinces, respectively.
Bayesian inference (A) and maximum likelihood (B) phylogenetic trees based on the concatenated mitochondrial Cytb and nuclear ITS-1 sequences. The number at the node denotes the Bayesian posterior probability (0.60 and greater only) and bootstrap percentage of maximum-likelihood analysis (60% and greater only). Dugesia ryukyuensis is used as the outgroup.
Median-joining haplotype network based on the concatenated mitochondrial Cytb and nuclear ITS-1 sequences. Each circle represents a specific haplotype, and its size proportional to its frequency. Different geographical populations are shown in different colors.
Mismatch distribution based on the concatenated mitochondrial Cytb and nuclear ITS-1 sequences.
Pairwise Fst values among Dugesia japonica populations.
Note: *P<0.05; The highest and lowest values are highlighted in bold.