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Polyphenol Content and Antioxidant Activity of Native Cultivar of Cannabis sativa L. of Pakistan

Polyphenol Content and Antioxidant Activity of Native Cultivar of Cannabis sativa L. of Pakistan

Hafiz Husnain Nawaz1*, Ayesha Ahsan2, Amir Afzal1, Muhammad Ashraf Sumrah1, Muhammad Jan1, Kashif Ali1, Muhammad Arsalan1 and Rizwan Latif3

1Center of Excellence of Olive Research and Training, Barani Agricultural Research Institute, Chakwal, Punjab Province, 48800, Pakistan; 2Department of Chemistry, Allama Iqbal Open University Sector H-8, Islamabad- 44000, Pakistan; 3Soil and Water Testing Laboratory, Talagang Road, Chakwal, Pakistan.

 
*Correspondence | Nawaz Hafiz Husnain, Center of Excellence of Olive Research and Training, Barani Agricultural Research Institute, Chakwal, Punjab Province, 48800, Pakistan; Email: h_husnain_012@yahoo.com 

ABSTRACT

Cannabis sativa is a high-value crop that grows naturally in Pakistan’s highlands. This is commonly known “Bhang” in Pakistan. This study is an attempt to recognize the C. sativa plant’s wonderful physiochemical qualities and socioeconomic benefits. Antioxidants abound in C. sativa extract, thanks to its high phenolic content. It’s a high-polyunsaturated acid crop that’s strong in antioxidants. Extraction from C. sativa seeds yielded a 33% yield of C. sativa oil, which was then analyzed for TPC and antioxidants in comparison to commercially available C. sativa oil. The Folin-Ciocalteu reagent and the α, α-diphenyl-β-picrylhydrazyl (DPPH) test were used to measure the total phenolic content of oil and leaf extracts. Bioactive components included in plant extracts, such as tocopherol, chlorophyll, carotenoids, phenolics, flavonoids, and other scavenging components, make them more potent. Thus, the 50µg/ml concentration plant extract solution has the lowest radical scavenging percentage, whereas the 1000µg/ml concentration plant extract solution has the highest DPPH percentage 80%. The results of the test reveal that the extract concentration had an influence on the assay’s absorbance, or, to put it another way, an increase in extract concentration produces a drop in absorbance, demonstrating an inverse relationship. The extract’s antioxidants inhibit DPPH, causing the absorbance to gradually diminish. Our study described that C. sativa have a wide range of volatile compounds with multiple range of physiochemical qualities and socioeconomic benefits so our study emphasizes on the future work with belief that C. sativa is a valuable crop that should be further investigated for commercial and culinary purposes.

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Pakistan Journal of Agricultural Research

September

Vol. 35, Iss. 3, Pages 477-577

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