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Perspectives of using Potassium on Agronomic, Bio Chemical and Yield Parameters of selected Cotton Varieties under Combined Stress of Salinity and Boron

PJAR_34_4_830-836

Research Article

Perspectives of using Potassium on Agronomic, Bio Chemical and Yield Parameters of selected Cotton Varieties under Combined Stress of Salinity and Boron

Muhammad Aftab1*, Aneela Riaz2, Ghulam Sarwar3, Muhammad Arif1, Qudsia Nazir1, Ifra Saleem1, Sarfraz Hussain1, Abid Ali4, Abid Niaz1, Fakhar Mujeeb4, Khalid Mahmood5 and Sarfraz Nawaz6

1Institute of Soil Chemistry and Environmental Sciences, AARI, Faisalabad, Pakistan; 2Soil and Water Testing Lab, Nankana Sahib, Pakistan; 3Depatment of Soil and Environmental Sciences, College of Agriculture, University of Sargodha, Sargodha, Pakistan; 4Soil Bacteriology Section, Agri. Biotechnology Research Institute, AARI, Faisalabad, Pakistan; 5Soil and Water Testing Lab, Toba Tek Singh, Pakistan; 6Pesticide Quality Control Laboratory, AARI, Faisalabad, Pakistan.

Abstract | Salt stress interrelates with other stresses as boron toxicity. However, the mechanism involve in salinity-boron interaction is still not well known. This research was carried out to evaluate the plants tolerance under combined stress of B and salt. In combined stresses of (B and Salt) causes oxidative stress in plants due to increase in production of the reactive oxygen species (ROS) including hydroxyl radicals (OH-), hydrogen peroxide (H2O2) and superoxide (O2-). However, on their side, efficient antioxidant defense system developed in plants, which having lower molecular weight of antioxidants. It also reduces (ROS) enzymatic activities including catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD). Under combined stress conditions agronomic, bio-chemical, quality and over all yield reduces, which is an alarming situation for agriculture sector. To assess the K effect on bio-chemical and agronomic characteristics of selected cotton crop varieties, a lysimeter study was carried out under combined stresses(Salinity and B) conditions. There were two varieties of cotton crop used in this research, such as, 1) FH-114, which was tolerant, and 2) NIAB-846 was sensitive to B-salinity stress. Sodium Chloride (NaCl) and Boric acid (H3BO3) were used to produce salinity and boron toxicity alone or in combined form and potassium (K) was applied as (K2SO4). K has helpful role under combined stresses (B and salinity) to improve cotton yield. According to the results, the agronomic and growth parameters improved by the application of K. But the toxicity of salinity-boron badly affects the yield and growth parameters in stresses conditions.


Received | June 22, 2021; Accepted | October 08, 2021; Published | October 31, 2021

*Correspondence | Muhammad Aftab, Institute of Soil Chemistry and Environmental Sciences, AARI, Faisalabad, Pakistan; Email: m.aftabjee@gmail.com

Citation | Aftab, M., A. Riaz, G. Sarwar, M. Arif, Q. Nazir, I. Saleem, S. Hussain, A. Ali, A. Niaz, F. Mujeeb, K. Mahmood and S. Nawaz. 2021. Perspectives of using potassium on agronomic, bio chemical and yield parameters of selected cotton varieties under combined stress of salinity and boron. Pakistan Journal of Agricultural Research, 34(4): 830-836.

DOI | https://dx.doi.org/10.17582/journal.pjar/2021/34.4.830.836

Keywords | Boron, Potassium, Salinity stress, Chlorophyll content, Cotton



Introduction

Globally, salinity is a major threat for food security (Syed et al., 2021; Majeed et al., 2010). Toxicity of B is related to salinity problem described by (Pandey et al., 2019). It has been stated by (Nabal et al., 1997), the B toxicity is a main problem in dry regions of the world, so which can diminish the growth of plants (Camacho et al., 2008). According to literature, B uptake mechanism is still ambiguous in saline conditions. The B translocation and absorption by passive diffusion may inhibited in salinity conditions and it also reduced transpiration rates. (Kronzucker et al., 2008) has been revealed that, most of the world’s soils are salt affected soils (approx. 30%) that make 1/3 of the world’s food. Generally, plants development and growth were gone through in two ways under saline conditions, (Munns and Tester, 2008), i) soil water potential decreases due to osmotic stress, and it also leads to limit the water uptake by plants; ii) excessively in uptake of different ions such as (B, Cl- and Na+) eventually have an effect on the various plants metabolical processes. It has been observed, (Ashraf, 2002), plant reactions to osmotic and ionic mechanisms in saline conditions, are extremely complex for metabolism processes and various gene networks. These forms of reactions primarily depend on different conditions such as, time duration (salts exposure to the roots of plant), severity of salinity and the inherent tolerance of salts in plant. Hasegawa et al., 2000) has been cited that, higher the concentration of (Cl-, B and Na+) nutrients, nutritional imbalance takes place in the plant cells, therefore, decline in assimilation of mineral/nutrients including manganese (Mn), potassium (K) and calcium (Ca) was observed. (Marschner, 1995) has been resulted that in leaf apoplasm, salts toxicity concentrations are increase that it may have adverse effects on leaf cells (dead), dehydration and turgor pressure losses etc. However, if Na+/ K+ ratio is higher than dehydration of cells occurred, Na toxicity concentrations cause hindrance in metabolical processes of plant and the activities of enzymes, (Booth and Beardall, 1991), and hence, these factors contribute towards less growth and low yield of crops. On the other hand, declining in agriculture’s production is due to salts accumulation in soils and irrigation water of low quality was used (which is also the major reason in less productivity of agriculture). Literally cited by (Grieve and Poss, 2000), in the arid regions, declining in yield and growth of plant is a rigorous hindering towards agriculture. So, it is not a final solution to get better the plant resistance to salinity conditions, which may decrease the salinization problem through irrigation by reducing inputs and also enhance stability in yield of subsistence agriculture, described by (Flowers and Yeo, 1995).

Potassium (K+) is a fundamental macronutrient and is a most abundant cation. It also performs vital role in several physiological processes of plant like transport of assimilates, photosynthesis and enzymes activation (Aftab et al., 2016). It influences plant physiological and biochemical activities, (Abbasi et al., 2014), which decrease growth and metabolical process of plant. It has also been found that, it (K) provides survival for plants, when K exposed to under various biotic and biotic environments. In addition, under certain environmental conditions, (K) is an important nutrient for obtaining high yield of crop, on the other hand, (Liebersbach et al., 2004; Qi and Spalding, 2004), it can be a limiting factor for crops such as water stress and salinity. It has been observed by (Rengel and Damon, 2008) that, illumination of mechanisms responses has main role in plants adaptation to potassium’s deficiency.

Cotton (Gossypium hirsutum L.) also called as “Queen of fiber”, and is the most important crop of Pakistan (Ali et al., 2019). It is mainly grown crop for both oilseed and fiber. Cotton is also grown globally, almost an area of 3106 kg ha-1 along with average yield of 695 kg ha-1 and provided productivity of 12968 thousand bales (Rana et al., 2020). Basically, the objective to plan this research was to estimate; i) the yield of two selected cotton varieties under combined stress of boron (B) and salinity, and ii) the role of applied potassium (K) on cotton growth.

Materials and Methods

Lysimeter experiment

To assess the influence of potassium (K) on yield, bio-chemical and agronomic parameters of selected cotton varieties (NIAB-846 and FH-114) stress of salinity-boron (in combination) stresses. Therefore, this lysimeter experiment was conducted at the Institute of Soil and Environmental Sciences (ISES), which is located at University of Agriculture (UAF), Faisalabad. Sieving of soil (5 mm mesh size) was done and the Lysimeter were filled with air-dried soil. The samples of soil were collected at normal depth about (0-15 cm) from soil surface. The texture of soil was sandy clay loam texture (0.68%) along with sand (51.5%), silt (27.75%) and clay (21.25%) fractions. Then, other analysis were also assessed as extractable potassium (K), total nitrogen (8.79 mg N kg-1) and available phosphorous (84 mg P kg-1), by (Watanabe and Olsen, 1965), and through making standard solutions. After that, the soil physico-chemical properties were calculated as OM (0.06%), electrical conductivity (1.41 dS m-1), pH (7.9), saturated percentage (31.5%) through following methods of (Moodie et al.,1959; Richards, 1954).

By following all protection measures of plants, tap water of good quality was used for irrigation purposes. For cotton crop, the nitrogen and phosphorous fertilizers was applied to soil at sowing time with recommended dose of N (197 kg ha-1 and P (94 kg ha-1. In lysimeter, the soil K concentration was calculated and added to soils. The remaining levels of K+ were applied to soil along with recommended doses and/or concentrations of B and salinity were also added to soil artificially as followed by [B (5 mM H3BO3) + salinity (140 mM NaCl) + (3 and 6 mM K+)]. In treatment (T1), the original soil (K+) was kept as control. Removal of two healthy and uniform seedlings was done after germination for suitable growth of remaining plants.

A set of ten (10) treatments including T1= Control (original soil K+ recommended dose of N and P), T2= (140 mM NaCl), T3= (5 mM B), T4= (140 mM NaCl + 5 mM B), T5= (140 mM NaCl + 3 mM K+), T6= (140 mM NaCl + 6 mM K+),T7= (5 mM B + 3 mM K+),T8= (5 mM B + 6 mM K+),T9= (140 mM NaCl + 5 mM B + 3 mM K+),T10= (140 mM NaCl + 5 mM B+ 6 mM K+) were added to soil at the time of sowing for both cotton varieties in three replications. For this study, the complete randomized design (CRD) under factorial arrangement was used.

The plants were harvested at maturity, and data relating to yield and growth of cotton varieties were recorded at different B and salinity levels, including (no. of branches plant-1, lint yield plant-1 (g), boll radius, internodal distance, no. of bolls plant-1 and height of plant) (Figure 1B). For the determination of chlorophyll contents of second top leaf before harvesting of crop hand held Minolta-502 chlorophyll meter was used.

Statistical analysis

The calculated data of parameters were taken. All parameters were subjected to analysis of variance (ANOVA) through a software (Statistix v. 8.1), given by (Analytical Software, USA). The significance of means of treatment was showed by lettering (alphabetically) and standard error (S.E). The means of treatment were compared through LSD test (least significant difference) at (5%) probability, and means that carrying same letters were considered non-significant statistically (p≤0.05) (Steel et al., 1997).

Results and Discussion

Due toincrease in B and salinity levels, the agronomic attributes of cotton crop were rigorously affected on the other hand, in saline soil, improvement was observed by the K applications. All physical parameters such as no. of branches plant-1, intermodal distance (Table 1), boll radius (Figure 1D), plant height and lint weight (Figure 1A), under boron toxicity and saline conditions. No. of flowers plant-1 (Figure 1C)were less as compared to those treatments where K+ was applied. The effect of K+ application was most favorable in those treatments where K+ was applied in both salt and B stress as compared to untreated treatments. Same trend was observed in all growth parameters in both cotton varieties.

 

Table 1: Agronomic characteristics of varieties of cotton at different levels of boron (B) and salinity.

Treatment

FH-114

NIAB-846

Plant height (cm)

No. of branches plant-1

Intermodal distance (cm)

Plant height (cm)

No of branches plant-1

Intermodal distance

T1

117.6cd

10c

4.2d

113.4b

09b

4.1cd

T2

111.3i

09de

3.8e

100.4g

09bc

3.3e

T3

118c

11bc

4.5c

113.9b

10b

4.4bc

T4

112.7h

08e

3.6f

100.4g

06e

3.3e

T5

115.2f

09d

4.0e

104.7e

08c

3.4e

T6

116.6de

10c

4.3d

111.2c

09bc

4.0d

T7

119b

12ab

4.7b

115.1b

11a

4.6b

T8

120.6a

12a

5.2a

117.1a

11a

5.0a

T9

114.1g

09de

3.9e

102.3f

07d

3.4e

T10

116ef

10c

3.9e

109d

08c

3.8d

LSD

0.9804

0.7455

0.1844

1.702

0.9396

0.3514

Values with the same letter are not statistically significant(p<0.05)

 

Under toxicity of B and saline stress, reduction was observed in chlorophyll contents. The chlorophyll contents showed increase by K applications illustrated in (Figure 2). NIAB-846 showed comparatively low response, while FH-114 showed maximum response to applied potassium. In FH-114 and NIAB-846, there was 45.8 and 40% decrease, respectively, in T4 (140 mM NaCl + 5mM B) was observed as compared to T1 where (original level of k++ recommended dose of N and P). Potassium application was more effective to combat salinity and boron toxicity. The higher level of potassium application increases lint weight, no. of bolls and chlorophyll contents. Combined salt and B stress reduces significantly the chlorophyll contents (Figure 2). So, results proved that the combined effect of treatment B and salinity interaction/toxicity (in combination) was harmful to plants as compared

 

 

to other treatments. NIAB-846 showed reduces results in all parameters as compared to than FH-114.

In salinity, decrease in yield and growth of plant are due to salinity stress. This reason of salinity changes all the characteristics of crops involving (biochemical, physiological, morphological and agronomic attributes). In all growth parameters reducing trend was observed under saline and boron stress. Adcock et al. (2007) confirmed that frequently B is present in higher concentration, and it shows a relationship with salinity (in combination). (Aftab et al., 2015) showed, several different results have been present on the effect of B-salinity interaction (Ashraf, 2009) declared that, cotton plant response is variable at different growth stages. Some cultivars were proved tolerant to saline conditions at seedling stage, but during later stages, it was not tolerant to salinity. (Hu et al., 2008) declared that,decreased no. of branches plant-1 and height of plantfor fertilizer application might be because of time being fertilizer stress. Under medium saline conditions osmotic adjustment of plant might be easy as compare to high saline conditions (Qados, 2011). Early growth stages of plants are salt senstive that is the main reason of decreased agronomic, biochemical and quality parameters of crops (Acosta-Motos et al., 2017). In salinty conditions, raised internodal distance and decrease in no. of branches per plantare observed and associated to peroxidation of lipid along with the reactive oxygen species (ROS) addition (Klein, 2012). Potassium (K+) has a main function in salt tolerance plant which is potassium (K+) uptake is decreased by Na+ (Fox and Guerinot, 1998; Aftab et al., 2016). Moreover, Rahnama and Ebrahimzadeh (2004) has also declared, K performs an essential role in most of the physiological characteristics of plant such as, transport of sugar, enzymes activation, osmotic adjustment, starch synthesis, synthesis of ATP and protein synthesis etc.

Higher the salinity stress, markedly reduction was noted, (Akcin and Yalcin, 2016; Nawaz et al., 2010), in leaves chlorophyll contents. It is documented in literature by (Ali et al., 2004) that decline in leaves chlorophyll contents was the reason of b-carotene degradation and, (Gomes et al., 2011), the adverse effects of accumulation of salts in ions. By the application of potassium sulfate chlorophyll contents improved. Hussein et al. (2014) stated, similar results obtained in Jojoba plants. Potassium fertilizers under saline conditions enhanced photosynthetic pigments by increasing the potassium ion and reducing the sodium ions (Fayez and Bazaid, 2014; Hu et al., 2008). In the previous studies similar results were found by some other scientists (Arshadullah et al., 2014; Sharma and Minhas, 2005).

Conclusion and Recommendations

The B and salinity interaction has harmful effects on the yield and growth of two cotton varieties like NIAB-846 and FH-114. The cotton variety (FH-114) towards K applications responds positively as compared to (NIAB-846) variety, as (FH-114) variety obtained more potassium (K). In combined stress of boric acid (H3BO3) and sodium chloride (NaCl), K application was added into soil in the form of (K2SO4), FH-114 has more potential to enhance the yield and growth as compared to the variety (NIAB-846). It has been concluded that the K application under combined stress of salts and boron showed positive results in growth, biochemical and yield parameters of both varieties of cotton.

Acknowledgment

This article and study trial makes possible by Ayub Agriculture research Institute (AARI), Faisalabad and University of Agriculture (UAF), Faisalabad.

Novelty Statement

Toxicity of salinity-boron badly affects yield and growth parameters of cotton in stress conditions.

Author’s Contribution

Muhammad Aftab: Conception and design of the work and conduction of experiment.

Aneela Riaz: Drafting and technical assistance.

Ghulam Sarwar: Supervision and technical assistance at every step.

Muhammad Arif and Qudsia Nazir: Final editing and proof-reading Interpretation of data and excel work for graphs making.

Ifra Saleem: Helped in lab. Work.

Sarfraz Hussain and Abid Ali: Interpretation of data and excel work for graphs making.

Abid Niaz and Fakhar Mujeeb: Assisted in write up and final editing of manuscript.

Khalid Mahmood: Elaborated results and discussion.

Sarfraz Nawaz: Statistical analysis of data.

Conflict of interests

Authors announce no conflict of interest.

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