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Parentage and Genetic Diversity Studies on Pangolin (Manis javanica) using Novel Microsatellite Markers

Parentage and Genetic Diversity Studies on Pangolin (Manis javanica) using Novel Microsatellite Markers

Hui-Ming Li1, Bi-Ze Yang1, Xue-Jun Jin1,2, Lin-Miao Li1, Hai-Ying Jiang1
Xiu-Juan Zhang1 and Jin-Ping Chen1*

1Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangdong Academy of Science, Guangzhou 510260, China
2Dongguan Institute of Qingfengyuan Animal Medicine, Dongguan, Guangdong, China

*      Corresponding author:


The Malayan pangolin (Manis javanica) is an endangered mammal species exhibiting scales characteristics. The conservation and management of this species could benefit from a better understanding of its genetic diversity and structure. In this study, 24 novel SSRs were isolated from full-length transcriptome and they were used for assessing of parent-offspring relationship for M. javanica. All SSR markers were highly polymorphic with a mean of 6 alleles per locus, ranged from 2 to 10 alleles. The average polymorphism information content (PIC) was 0.62. The observed (HO) and expected average heterozygosity (HE) value was 0.56 and 0.67 tested in 39 individual samples. For parentage testing, the allele frequency date-based indicated that the combined exclusion probability values were over 99.99% when 9 SSRs were used. This study demonstrated that the microsatellite-based approach could be effectively utilized for parentage analysis in pangolins and that it has a significant application in selective breeding in endangered and valuable mammalian species.

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Pakistan Journal of Zoology


Vol. 54, Iss. 5, Pages 2003-2500


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