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Oral SS-14 DNA Vaccine is More Potent than Oral SS-28 DNA Vaccine in Promoting Rat Lactation

Oral SS-14 DNA Vaccine is More Potent than Oral SS-28 DNA Vaccine in Promoting Rat Lactation

Yan-Guo Han1,2, Jun-Hua Ye1, Qin-Tao Zhao1, Yong-Jie Huang2, Kai Li2, Yong-Fu Huang2 and Li Xu1,*

1Nanchang Police Dog Base of Ministry of Public Security, Nanchang 330100, China
2Chongqing Key Laboratory of Forage and Herbivore, Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization, College of Animal Science and Technology, Southwest University, Chongqing 400715, China

*      Corresponding author: xuli7403112018@126.com

 

Fig. 1.

Map of construction of oral somatostatin (SS) DNA vaccines encoding different lengths somatostatin and delivered by attenuated Salmonella choleraesuis C500. A, Map of construction of oral SS-14 DNA vaccine fused tPA signal peptide and CpG adjuvant named C500 (ptCS/2SS-14-asd); B, Map of construction of oral SS-28 DNA vaccine fused tPA signal peptide and CpG adjuvant named C500 (ptCS/2SS-28-asd); C, Map of construction of oral empty vector vaccine fused CpG adjuvant named C500 (pCpG-asd).

Fig. 2.

Identification of recombinant SS DNA vaccines encoding different lengths somatostatin and empty vector vaccine by PCR. Lanes 1, 2 and 3, C500 (ptCS/2SS-28-asd), C500 (ptCS/2SS-14-asd) and C500 (pCpG-asd) recombinant DNA vaccines were amplified with T7 and BGH primers; Lane M, DL5000 DNA marker. Three bands shown in Lanes 1, 2 and 3 are tPA-CpG-HBsAg-S-2SS-28-FLAG (1086 bp), tPA-HBsAg-S-2SS-14-FLAG (1002 bp) and CpG (178 bp) fragment, respectively.

Fig. 3.

Anti-SS antibodies titers in T1, T2 and C groups immunized with C500 (ptCS/2SS-14-asd), C500 (ptCS/2SS-28-asd) and C500 (pCpG-asd), respectively at weeks 3 (first day after childbirth) and 7 (week 4 after childbirth) after primary immunization. Vaccinations were respectively orally given at weeks 0, 3 and 6 weeks after primary immunization. Data are presented as means ± SD. *p < 0.05.

Fig. 4.

Serum GH (A), PRL (B) and IGF-1 (C) concentrations (ng/mL, uIU/mL and ng/mL, respectively) in T1, T2 and C groups immunized with C500 (ptCS/2SS-14-asd), C500 (ptCS/2SS-28-asd) and C500 (pCpG-asd), respectively at weeks 0 (before mating), 5 (week 2 after childbirth) and 7 (week 4 after childbirth) after primary immunization. Data are presented as means ± SD. *p<0.05.

Fig. 5.

Body weight (g) of offspring rats in T1, T2 and C groups at weeks 0 (first day after childbirth), 2 (week 2 after childbirth) and 4 (week 4 after childbirth) after birth. Data are presented as means ± SD. *p < 0.05.

Pakistan Journal of Zoology

October

Vol. 51, Iss. 5, Pages 1599-1997

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