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Optimization Study of Lipolytic Enzyme from Bacillus cereus, PCSIR NL-37

Optimization Study of Lipolytic Enzyme from Bacillus cereus, PCSIR NL-37

Haniya Mazhar1, Naaz Abbas2, Tehseen Zamir3, Zahid Hussain1, Syed Shahid Ali4*

1Institute of Industrial Biotechnology,, GC University, Lahore-54000, Pakistan
2FBRC, PCSIR Labs. Complex, Lahore-54600, Pakistan
3Punjab University Medical Centre, Quaid-e-Azam Campus, Lahore-54590, Pakistan
4Department of Zoology, University of the Punjab, Quaid-e-Azam Campus, Lahore-Pakistan.

*Corresponding Author: Syed Shahid Ali, shahid.zool@pu.edu.pk

 

ABSTRACT

In this study, the optimum growth conditions and lipolytic enzyme activity shown by the bacterial isolate (PCSIR NL-37) from the soil of Lahore (Punjab, Pakistan) are being reported. Lipolytic bacterial strains (100) isolated from soil and water samples from oil industry, mobile oil filling areas and kitchen wastages were screened on tributyrin agar and rhodamine agar plates. The strain PCSIR NL-37 was identified as Bacillus cereus following morphological, biochemical and molecular characterization. Best medium for lipase production was olive oil and mustard oil cake and the best carbon sources were fructose and maltose whereas yeast extract was found to be the best nitrogen source, showing 55U/mL enzyme activity. The best inoculum size for the growth of this strain was 4-5%. Optimum pH was 8 and best temperature was 40oC for the reported strain. Addition of divalent Mg2+ and Ca2+ ions resulted in 2-fold increase in enzyme activity while SDS completely inhibit the lipase activity to zero. 
 

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Punjab University Journal of Zoology

December

Vol. 34, Iss. 2, pp. 105-238

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