Cotton is a major crop of Pakistan after wheat and it occupies the largest area in Pakistan compared to other crops. Cotton is cultivated in Sindh on more than 1 million acres. Two popular varieties of cotton are grown in Sindh. The aim of the present study was to determine the diversity of plant parasitic and soil nematodes associated with cotton plants in Sindh. During 2017–2018 extensive surveys were conducted at the time of cropping and at harvesting and soil root samples were collected from different fields in the cotton producing regions of five districts of Sindh viz., Sanghar, Mirpurkhas, Umerkot, Mityari and Tando Allahyar. The analysis of samples resulted in the identification of one new species of soil nematode viz., Acrobeloides gossypii n. sp., along with three new record species viz., Tylenchorhynchus ewingi Hopper, 1959; T. crassicaudatus Williams, 1960 and Pratylenchus pseudofallax Café-Filho & Huang, 1989. Description, measurements and illustrations of these species are incorporated herein.