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New Genetic Evidence from Three Keel-Backed Liza Species Based on DNA Barcoding Confirms Morphology-Based Identification

New Genetic Evidence from Three Keel-Backed Liza Species Based on DNA Barcoding Confirms Morphology-Based Identification

Lu Liu1, Sher Khan Panhwar2, Tianxiang Gao3, Zhiqiang Han3, Chunhou Li4, Dianrong Sun4 and Na Song1,*

1FisheriesCollege, Ocean University of China, Qingdao, 266003, People’s Republic of China
2Center of Excellence in Marine Biology, University of Karachi, Karachi, 75270, Parkistan
3Fishery College, Zhejiang Ocean University, 266003, People’s Republic of China
4South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510003, People’s Republic of China

*      Corresponding author: songna624@163.com

ABSTRACT

Three Liza species exhibiting a dorsal keel have been identified based on morphology: Liza affinis, Liza carinata, and Liza klunzingeri. To confirm the validity of the species by molecular methods, we sequenced a fragment of the cytochrome oxidase subunit I (COI) gene of mitochondrial DNA of Liza affinis and Liza klunzingeri from coastal waters of China and Pakistan, respectively. Sequences of L. carinata were obtained from GenBank. A neighbor-joining tree showed the specimens to form a strong monophyletic group. The mean within-species genetic distances for L. affinis, L. carinata, and L. klunzingeri were 0.26%, 0.52%, and 0.47%, respectively. The mean genetic distances among L. affinis, L. carinata, and L. klunzingeri ranged from 12.22% to 14.74%, higher than the threshold for species delimitation. The results confirmed the taxonomic validity of the three Liza species and verified that the COI gene could provide effective DNA barcoding for identification.
 

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Pakistan Journal of Zoology

June

Vol. 53, Iss. 3, Pages 801-1200

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