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Negative Association of HLA-DRB1*11 and HLA-DRB1*12 Alleles with Aeroallergy Patients Visiting Allergy Centre (NIH), Islamabad, Pakistan

Negative Association of HLA-DRB1*11 and HLA-DRB1*12 Alleles with Aeroallergy Patients Visiting Allergy Centre (NIH), Islamabad, Pakistan

Madiha Hashmi1, Abid Hussain1, Shafiq ur Rehman1, Farida Ahmed2, Shahbaz Aslam3,Nadeem Afzal4 and Zaigham Abbas1,*

1Department of Microbiology and Molecular Genetics, University of the Punjab, Quaid-e-Azam Campus, Lahore, Pakistan
2Allergy Centre, National Institute of Health, Islamabad, Pakistan
3Institute of Biochemistry and Biotechnology, University of the Punjab, Quaid-e-Azam Campus, Lahore, Pakistan
4Department of Immunology, University of Health Sciences, Lahore, Pakistan

*      Corresponding author: zaigham.mmg@gmail.com

Fig 1

HLA typing using PCR. M, DNA 1kb marker; C, positive controls for allele of interest; A, PCR with primers of internal control GAPDH (PCR product size = 576bp); B, PCR with primers of allele of interest; Allele of interest in a, HLA-DRB1*11 (PCR product size = 176bp); Allele of interest in b, HLA-DRB1*12 (PCR product size = 248bp); Lanes 1 to 17, Samples of aero-allergic patients.

Fig 2

Percentage prevalence of HLA-DRB1*11 (A) and HLA-DRB1*12 (B) amongst aero-allergy cases reported at Allergy Centre, NIH, Islamabad and healthy controls.

Pakistan Journal of Zoology

June

Vol. 50, Iss. 3, Pages 799-1198

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