Molecular Study on Field Evolved Resistance of Red Palm Weevil (Rhynchophorus ferruginous) and its Management through RNAi
Jam Nazeer Ahmad1,2*,Mujahid Manzoor1, Zubair Aslam3 and Samina Jam Nazeer Ahmad1, 2
Demonstration of the injection of dsRNA in the 5th, 10th instar larvae and adults of R. ferruginous.
P450 expression through PCR in several R. ferruginous larval instars (L1-1st instar, L3- 3rd instar, L5- 5th instar, L7- 7th instar and L10- 10th instar), male (M) and female (F).
P450 down regulation in 10th larval instars (L10) and its knockdown influence via microinjection of dsRNA Conc. (µg/insect).
Expression of P450 proteins by using ant-P450 antibodies.
Expression of P450 gene through qRT-PCR in male, female and larva of R. ferrugineus. (*P<0.05, **P<0.01 and ***P<0.005).
Beta-actin expression in R. ferrugineus larvae (10th instar and Male and Female from left to right) after the application of dsRNA-gfp and dsRNA-P450
Mortality percentage of R. ferrugineus population in response to Pesticide (Cypermethrin) and ds RNA P450 application. SL= “Susceptible larvae”- SM= “Susceptible male”- SF= “Susceptible female”- RL= “Resistant larvae”, RM= “Resistant Male”- RF= “Resistant Female”-, FL= Field larva, FM=Field Male and FF=Field Female (Field population of Sindh province was used. Mortality was observed after 48h of treatment. (*P<0.05, **P<0.01 and ***P<0.00