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Molecular Identification of Pakistani Lathy Rock Pigeon using Cytochrome B Gene

Molecular Identification of Pakistani Lathy Rock Pigeon using Cytochrome B Gene

Sehrish Firyal1,*, Ali Raza Awan1, Muhammad Umair Latif1, Muhammad Tayyab1, Muhammad Wasim1, Shagufta Saeed1 and Imaad Rashid2

 

1Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Civil Lines, Outfall Road, Lahore
2Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad

*     Corresponding author: sehrishfiryal@uvas.edu.pk

ABSTRACT

Single nucleotide polymorphism (SNPs) analysis is a powerful tool for taxonomic and phylogenetic analysis. These are bi-allelic molecular markers, easy to interpret and uniformly distributed within genomes. In this study cytochrome b (Cytb) gene based SNPs analysis was employed to genetically characterize the Pakistani domestic breed Lathy pigeon. Cytb haplotype for local domestic breed was developed. Homology analysis of Cytb gene revealed Columbia livia as closest homologue of Pakistani Lathy pigeon with five novel SNPs. Phylogenetic analysis of domestic lathy pigeon indicated the monophyletic relation with C. livia. We are reporting for the first time, the novel SNPs in the Cytb gene of local domestic pigeon breed that might act as panel of molecular marker for the identification of Pakistani lathy pigeon.
 

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Pakistan Journal of Zoology

October

Vol. 52, Iss. 5, Pages 1631-2026

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