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Molecular Identification of Lactic Acid Bacteria from Black Soldier Fly Larvae Reared on Different Substrates

Molecular Identification of Lactic Acid Bacteria from Black Soldier Fly Larvae Reared on Different Substrates

Aulia Nurul Saputri, I Komang Gede Wiryawan, Dilla Mareistia Fassah*, Dewi Apri Astuti 

Department of Nutrition and Feed Technology, Faculty of Animal Science, IPB University, Bogor, 16680, Indonesia.

*Correspondence | Dilla Mareistia Fassah, Department of Nutrition and Feed Technology, Faculty of Animal Science, IPB University, Bogor, 16680, Indonesia; Email: dilla_mareistia@apps.ipb.ac.id  

ABSTRACT

Usage of antibiotic growth promoters (AGP) in livestock causes certain antibiotic resistance. Probiotic may be used as an alternative for AGP by providing microorganisms that have a health benefit for the host. This study was to identify the molecular characteristics of LAB isolated from the digestive tract of black soldier fly (BSF) larvae on different substrates as probiotic candidates. The LAB from the digestive tract of BSF larvae in chicken manure and palm kernel meal substrates was isolated and taxonomically identified using the 16S rRNA sequence homology and molecular identification. The LAB isolates were also tested for antimicrobial and hemolysis activities. Five isolates from BSF larvae reared on chicken manure substrate and three isolates from BSF larvae reared on palm kernel substrate showed a gram-positive bacteria characteristic. The LAB isolates from BSF larvae reared on chicken manure and palm kernel meal substrate showed no different in inhibition zones against pathogenic bacteria (E. coli and S. typhimurium). All isolates showed no hemolysis activity. Three isolates (A3, A4, and B1) are molecularly identified as Enterococcus faecalis which showed a potency as probiotic candidate. We conclude that Enterococcus faecalis is a potential probiotic that can be isolated from BSF larvae.

Keywords | 16S rRNA Gene; BSF Larvae; Chicken Manure; Lactic Acid Bacteria; Palm Kernel Meal 

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Advances in Animal and Veterinary Sciences

1

Vol. 11, Iss. 1, Pages 1-188

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