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Molecular Identification and Prevalence of Ehrlichia canis and Rhipicephalus sanguineus (Acari: Ixodidae) Infecting Pet Dogs in Wenzhou, China

Molecular Identification and Prevalence of Ehrlichia canis and Rhipicephalus sanguineus (Acari: Ixodidae) Infecting Pet Dogs in Wenzhou, China

Houqiang Luo1*, Yanfang Lan2, Ping Gan3, Wenjun Zhou4, Meng Wang1
Bing Hu5, Zhuning Zhang5, Yu Bai1* and Kun Li6*

1College of Animal Science, Wenzhou Vocational College of Science and Technology, Wenzhou 325006, People’s Republic of China
2Wuhan Business University, Wuhan 430056, People’s Republic of China 
3Jiangxi Animal Disease Prevention and Control Center, Nanchang 330096, People’s Republic of China
4Qilu Animal Health Products Co., LTD., Jinan 250100, People’s Republic of China
5Agriculturral Service Center of Street Office of Datang affiliated to The People’s Government of Zhuji Municipality, Zhuji 311801, People’s Republic of China
6College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China.
 
Houqiang Luo, Yanfang Lan and Ping Gancontributed equally to this work.

*      Corresponding author: chviolet1984@sina.com; baiyu106@163.com; lik2014@sina.com

Fig. 1.

Phylogenetic tree for R. sanguineus based on partial COI gene sequences. Phylogenetic tree based on partial COI gene sequences of R. sanguineus was constructed using the neighbor-joining method with Kimura two-parameter analysis and bootstrap analysis of 1000 replicates. The numbers on the branches indicate the percentage of replicates that reproduced the topology for each clad. The green rhombi indicate the sequences acquired from the current study (Ascaris suum was used as an outgroup).

Fig. 2.

Phylogenetic tree for E. canis based on partial dsb gene sequences. Phylogenetic tree based on partial dsb gene sequences of E. canis was constructed by employing the neighbor-joining method with Kimura two-parameter analysis and bootstrap analysis of 1000 replicates. The numbers on the branches indicate the percentage of replicates that reproduced the topology for each clads. The red rhombi indicate the sequences acquired from the current study (Drosophila melanogaster was used as an outgroup).

Pakistan Journal of Zoology

October

Vol. 53, Iss. 5, Pages 1603-2000

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