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Molecular Identification and Pathological Characteristics of NPV Isolated from Spodoptera litura (Fabricius) in Pakistan

Molecular Identification and Pathological Characteristics of NPV Isolated from Spodoptera litura (Fabricius) in Pakistan

Jam Nazeer Ahmad1,2,*, Rashid Mushtaq1, Samina Jam Nazeer Ahmad1,2, Sumaira Maqsood3, Ishita Ahuja4 and Atle M. Bones4

1Intergrated Genomics Cellular Developmental and Biotechnology Lab, Department of Entomology, University of Agriculture, Faisalabad
2Plant Stress Physiology and Molecular Biology Lab, Department of Botany, University of Agriculture, Faisalabad
3Institute of Agricultural Sciences, University of the Punjab, Lahore
4Department of Biology, Norwegian University of Science and Technology, NO-7491 Trondheim, Norway

*      Corresponding author: jam.ahmad@uaf.edu.pk

 

ABSTRACT

The cotton army worm Spodoptera litura Fabricius (Lepidoptera: Noctuidae) is a destructive pest of various field crops and vegetables in Pakistan. Development of biopesticide is an attractive strategy to minimize the problems of pest resistance, environmental pollution and human health concerns. The isolates of S. litura nucleopolyhedroviruses (SltNPV) were collected from infected larvae fed on natural cotton crops. The NPV was isolated from the larvae and viral occlusion bodies (VOBs) were detected using a light microscope. The toxicity of native isolates against S. litura also studied by testing different concentrations (1 x 102 POB (Polyhedral occlusion bodies) mL-1- 1 x 1010 POB mL-1) from the occlusion bodies produced from NPV isolates against 2nd, 3rd, 4th and 5th instar larvae of S. litura. The rapid and sensitive polymerase chain reaction (PCR) technique was used for the molecular detection of NPV gene from native NPV diseased insect. Multiple sequence alignment and phylogenetic analysis were performed to compare SlNPV- FSD15 based on Lef-8 with other Lef-8genes sequences clearly showed that our SlNPV-FSD15 isolate belongs to Spodoptera litura associated NPVs. The biological activities of this NPV isolates were investigated under laboratory condition. The highest mortality of S. litura was observed at early instars. Against second instars of S. litura, LC50 values of NPV isolate ranged from 1.92×103 to 3.64×103OB/ml with LT50 values of 69.30 h to 72.80 h, respectively. This study showed highly effectiveness and provides an opportunity to cut down the use of synthetic approaches and develop safe biological/microbial insecticides from NPV isolates, which in future may effectively control S. litura.
 

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Pakistan Journal of Zoology

December

Vol. 50, Iss. 6, Pages 1999-2398

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