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Molecular Identification and Characterization of Fruit Flies of Genus Bactrocera (Diptera: Tephritidae) in Pakistan

Molecular Identification and Characterization of Fruit Flies of Genus Bactrocera (Diptera: Tephritidae) in Pakistan

Jam Nazeer Ahmad1*, Taiba Sharif3, Samina J.N. Ahmad2, Sumaira Maqsood4 and Fariha Zafar3 

1Department of Entomology, University of Agriculture, Faisalabad
2Department of Botany, University of Agriculture, Faisalabad
3Department of Zoology, University of Agriculture Faisalabad
4Institute of Agricultural Sciences, Punjab University, Lahore 

* Corresponding author: jam.ahmad@uaf.edu.pk

 

Fig. 1.

Multiple alignments of DNA sequences through CLUSTAL OMEGA from DNA amplification of 3 Pakistani Bactrocera species, B. cucurbitae 180319-003 (MK296116) isolate FSD-03, B. dorsalis 180319-004 (MK296118)isolate FSD-18 and B. zonata 180319-016 (MK296117) isolate FSD-16 with NCBI GenBank sequences, B. Cucurbitae (KJ753953.1), B. dorsalis (KF998634.1) and B. zonata (KP851002.1)

Fig. 2.

Phylogenetic analysis by Maximum Likelihood Method produced from DNA sequences of 3 Bactrocera species using mitochondrial Cytochrome oxidase I (mCOI) gene based primers. (A) B. cucurbitae 180319-003 (MK296116) (B) B. zonata 180319-016 (MK296117) (C) B. dorsalis 180319-004 (MK296118)

Pakistan Journal of Zoology

October

Vol. 51, Iss. 5, Pages 1599-1997

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