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Molecular Detection of Hepatitis E Virus in Layer Chickens: A Possible Public Health Risk in Pakistan

Molecular Detection of Hepatitis E Virus in Layer Chickens: A Possible Public Health Risk in Pakistan

Tahir Iqbal1, Umer Rashid1*, Naveed Shahzad2, Amber Afroz1Muhammad Faheem Malik3 and Muhammad Idrees4

 

1Department of Biochemistry and Biotechnology, University of Gujrat, Gujrat (50700), Pakistan
2School of Biological Sciences, University of the Punjab, Lahore (54000), Pakistan
3Department of Zoology, University of Gujrat, Gujrat (50700), Pakistan
4Hazara University, Mansehra, Khyber Pakhtunkhwa, Pakistan

*      Corresponding author: umer.rashid@uog.edu.pk

 

Fig. 1.

2% agarose gel showing amplifications of ORF1 and ORF 2 of aHEV genome in bile samples. (A) Helicase (ORF1) amplification (186 bp, arrow); Lane 1: PT7B; lane: PT9B; lane PT10B; lane 4: PT11B; lane 5: PT12B; lane 6: PT13B; lane 7: PT14B; lane 8: PT16B; lane 9: positive control; lane 10: 1 kb plus DNA marker; (B) ORF2 amplification (280 bp, arrow); Lane 1: PT7B; lane 2: PT9B; lane PT10B; lane 4: PT11B; lane 5: PT12B; lane 6: PT13B; lane 7: PT14B; lane 8: PT16B; lane 9: positive control; lane 10: negative control; M: 1 kb plus DNA marker.

Fig. 2.

The histological examination of liver (upper panel) and spleen (lower panel) tissues of aHEV positive layer chickens PT12 and PT16; (A) PT12 liver histology showing lymphocytic infiltration in liver tissues causing portal phlebitis and periphlebitis (arrows) ; (B) PT16 liver histology with lymphocytic infiltration (arrow heads) ; granulocytes (arrows) and multifocal necrosis (MNC); (C) PT12 spleen histology showing infiltration of monocytes (Mc); granulocytes (arrows); (D) PT16 spleen histology showing infiltration of basophils (Bph) and other large cells (arrows). The images were observed under 100X magnifications.

Fig. 3.

Identification of Pak aHEV strains (♦) on the basis of partial helicase domain (ORF1) nucleotides sequence. Neighbor-Joining method with 1000 bootstrapping replicates was used. GenBank accession number isolate/genotype (host) is shown for each member.

Fig. 4.

Identification of Pak aHEV strains (♦) on the basis of partial capsid protein (ORF2) nucleotides sequence. Neighbor-Joining method with 1000 bootstrapping replicates was used. GenBank accession number isolate/genotype (host) is shown for each member.

Pakistan Journal of Zoology

December

Vol. 51, Iss. 6, Pages 1999-2399

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