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Molecular Cloning and Function Characterization in Feeding of Neuropeptide Y in Gibel Carp, Carassius auratus gibelio

Molecular Cloning and Function Characterization in Feeding of Neuropeptide Y in Gibel Carp, Carassius auratus gibelio

Luo Lei1, Xingxing Deng1, Dengyue Yuan2, Zonglin Zheng1, Chengke Zhu1, Hui Luo1, Baohai Li3, Hua Ye1,* and Chaowei Zhou1,3,*

1College of Animal Sciences, Southwest University, Chongqing 402460, People’s Republic of China
2Department of Aquaculture, College of Life Sciences, Neijiang Normal University, Neijiang, Sichuan, 641000, People’s Republic of China
3Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, Tibet 850002, People’s Republic of China

Luo Lei and Xingxing Deng have contributed equally to this work.

*      Corresponding authors: 442589528@qq.com; 421807518@qq.com

 

Fig. 1.

The cDNA coding sequence of Carassius auratus gibelio npy. The shaded boldface letters stand for the mature peptide. * Indicates the stop codon.

Fig. 2.

Amino acid sequence alignment of NPY. The shadowed regions are conserved in the listed peptides. * Indicates identical amino acids.

Fig. 3.

The phylogenetic tree of vertebrate NPY. Scale bar indicates the substitution rate per residue. Numbers at nodes indicate the bootstrap value, as percentages, obtained per 1000 replicates. GenBank accession numbers: Typhlonectes natans (AAD48033), Xenopus laevis (NP_001081300), Ambystoma mexicanum (AAT66407), Bos taurus (AAR37328), Homo sapiens (AAA59944), Torpedo marmorata (AAA49281), Oncorhynchus mykiss (AAB25269), Salmo salar (BAH24101), Gadus morhua (AAX19943), Siniperca chuatsi (ABS83815), Dicentrarchus labrax (CAB64932), Paralichthys olivaceus (BAB62409), Ictalurus punctatus (AAF71617), Danio rerio (NP_571149), Carassius auratus (AAA49186), Spinibarbus sinensis (ABE73783).

Fig. 4.

Relative expression of npy mRNA in tissues of gibel carp. Error bars represent standard error of the mean. Tep, telencephalon; Hyp, hypothalamus; Mep, mesencephalon; Ceb, cerebellum; Myp, myelencephalon; Pi, pituitary; Hk, head kidney; Tk, trunk kidney; Li, liver; Fg, foregut; Mg, midgut; Hg, hindgut; He, heart; Sp, spleen; Gi, gill; Te, testis; Ov, ovary. Re, red muscle; Wh, white muscle; Sk, skin; Ey, eye.

Fig. 5.

Postprandial changes in npy mRNA expression in the hypothalamus of gibel carp. The preprandial (-3, -1 and 0 h) and postprandial groups (+3 and +1 h) that differed significantly are indicated by different letters above the bars. Asterisks represent significant differences between the fed and unfed groups at the same time point. **P < 0.01.

Fig. 6.

Effects of fasting and refeeding on npy mRNA expression in the hypothalamus of gibel carp. Bars with different letters represent significant differences between experimental groups. Asterisks represent significant differences between the groups at the same time point. **P < 0.01.

Pakistan Journal of Zoology

April

Vol. 51, Iss. 2, Pages 399-799

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