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Molecular Characterization of Plasmodium relictum in Four Common Bird Species in India

Molecular Characterization of Plasmodium relictum in Four Common Bird Species in India

Gautam Patra1*, Ana Sahara2, Sonjoy Kumar Borthakur1, Parthasarathi Behera3, Subhamoy Ghosh1, Apurba Debbarma4 and Seikh Sahanawaz Alam5

1Department of Veterinary Parasitology, Central Agricultural University, India
2Department of Veterinary Parasitology, Gadjah Mada University, Yogyakarta, Indonesia
3Department of Veterinary Physiology and Biochemistry, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram, India.
4Department of Veterinary Parasitology, College of Veterinary Sciences and Animal Husbandry, R.K. Nagar, Agartala, Tripura, India
5Department of Botany, Garhbeta College, Paschim Midnapore, West Bengal, India

*      Corresponding author: dr.gautampatra@yahoo.co.in

Fig. 1.

Trophozoite of P. relictum in the erythrocytes.

Fig. 2.

Identification on 1.5% agarose gel of 712 bp products of amplification of the cyt-b gene of P. relictum after colony PCR; L1–Negative; L2, L3–Positive amplification (712bp); M–100bp DNA ladder.

Fig. 3.

Distance matrix of cyt-b gene sequence of P. relictum with the available sequences from GenBank.

Fig. 4.

Phylogenetic tree of P. relictum isolates including the outgroup (P. falciparum).

Fig. 5.

Neighbour joining tree of P. relictum sequences without an out group.

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Pakistan Journal of Zoology

February

Vol. 53, Iss. 1, Pages 1-400

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