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Molecular Characterization of Heat Shock Protein 70-1 Gene of Capra aegagrus blythi

Molecular Characterization of Heat Shock Protein 70-1 Gene of Capra aegagrus blythi

Fehmeeda Fatima, Asif Nadeem* and Maryam Javed

Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan

Fehmeeda Fatima and Asif Nadeem contributed equally to this article.

*      Corresponding author: asifnadeem@uvas.edu.pk

 

ABSTRACT

Sindh ibex (Capra aegagrus blythi) is a vulnerable subspecies of wild goat (Capra aegagrus), which faced serious population threats. In the present study, HSP70.1 gene has been partially sequenced (1344 bp) to study genetic diversity and evolutionary characteristics of Sindh ibex. In this research, samples of Sindh ibex were collected from Kirthar National Park, Sindh, Pakistan, the gene of interest was amplified, PCR products were sequenced and data was analyzed. The results of genetic diversity indicated that Parsimony informative sites were found to be 11 while Singleton variable sites were not detected. The total number of haplotypes (h) was found to be 17 and haplotype diversity (Hd) was 0.862. Nucleotide diversity (pi) was calculated to be 0.00210 and Tajima’s D neutrality test for neutral mutation indicated a negative value. Moreover, the results of homology analysis showed maximum similarity with sequences of goat and sheep sequences (partial) and were found to be 99.93% and 99.35%, respectively which was further confirmed by constructing phylogenetic tree which indicated that Sindh ibex, domestic goat, and sheep share a common ancestor.
 

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Pakistan Journal of Zoology

August

Vol. 51, Iss. 4, Pages 1203-1598

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