Submit or Track your Manuscript LOG-IN

Molecular Characterization and Phylogeny of Panthera pardus (Common Leopard) in Pakistan

Molecular Characterization and Phylogeny of Panthera pardus (Common Leopard) in Pakistan

Usman Ijaz1, Muhammad Sajid Tahir2, Khalid Abdul Majeed3*, Shahid Iqbal4, Iffat Huma5, S. Firyal6, Ijaz Ahmed7, Shahid Chohan8 and Aamir Riaz Khan9 

1World Wildlife Fund, Ferozepur Road, Lahore, Pakistan

2Veterinary Diagnostic Laboratory, Johar Town, Lahore, Pakistan

3Department of Physiology, University of Veterinary and Animal Science, Lahore, Pakistan

4Serum Section, Veterinary Research Institute, Tufail Road, Lahore, Pakistan

5Quality Control Laboratory, Veterinary Research Institute, Tufail Road, Lahore, Pakistan

6Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Science, Lahore, Pakistan

7Imperial University, Lahore, Pakistan

8School of Biological Science, University of Punjab, Lahore, Pakistan

 

Email: khalid.majeed@uvas.edu.pk

ABSTRACT

The present study was designed to investigate the evolutionary relationship of the Panthera pardus subspecies found in Pakistan by exploring the partial DNA sequences of mitochondrial Cytochrome c Oxidase subunit I (COI) gene. Scat samples of 15 different known leopards were collected from Nathiagali, Khyber Pakhtunkhwa, Pakistan and Azad Jammu and Kashmir, Pakistan. After amplification with specific oligos, the amplicons of partial region of COI were subjected to sequencing, and then observed for single nucleotide polymorphisms (SNPs). SNPs were observed on three loci of the COI gene as compared to reference sequence of Panthera pardus pardus. The specimens found in Pakistan were found to be closely related to Panthera pardus orientalis (Amur Leopard in South eastern Russia) and Panthera pardus japonensis (North Chinese Leopard), with all of these three speculated to be evolved from Panthera pardus pardus in not-so-far past. Our findings are helpful in ascertaining the origin and closeness of leopard sub-species in Pakistan.

 

To share on other social networks, click on P-share. What are these?

Pakistan Journal of Zoology

December

Vol. 51, Iss. 6, Pages 1999-2399

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe