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Methanolic Extract of Pimpinella anisum L. Prevents Dementia by Reducing Oxidative Stress in Neuronal Pathways of Hypermnesic Mice

Methanolic Extract of Pimpinella anisum L. Prevents Dementia by Reducing Oxidative Stress in Neuronal Pathways of Hypermnesic Mice

Aamir Mushtaq1,2, Rukhsana Anwar1 and Mobasher Ahmad1,2,* 

1Punjab University College of Pharmacy, University of the Punjab, Allama Iqbal Campus Lahore, 
2Gulab Devi Institute of Pharmacy, Gulab Devi Educational Complex, Main Ferozpur Road Lahore, 54000 Pakistan

*  Corresponding author: ahmadmobasher@hotmail.com

ABSTRACT

Considering Alzheimer’s disease a universal challenge, this study was planned to utilize the bio-constituents of Pimpinella anisum dried seeds to check the oxidative neuronal decay in brain parts. Methanolic extract of Pimpinella anisum (meP.a) dried seeds was explored qualitatively and quantitatively for analysis of phytoconstituents along with anti-oxidant activity. Anti-amnesic activity was found by behavioral and biochemical studies for which male albino mice were grouped into seven groups. Mice were subjected to elevated plus maze, hole board and light dark paradigm on (day 7th and 8th), for behavioral studies after which their brain were removed by decapitation for biochemical assessment. Results indicated the existence of total phenols (170.14 ± 1.16 mg of GAE/g of extract), flavonoids (87.67 ± 0.67 mg of RE/g of extract), tannins (194.04 ± 1.79 mg of TAE/g of extract) and anti-oxidants (IC50 value = 59.60 µg/ml found by DPPH method) in plant extract. Higher inflexion ratio (0.25 ± 0.05), most of time spent in dark area (158.34 ± 4.88 sec), increased no of hole pokings (34.16 ± 2.01), significant (P < 0.001) elevation in superoxide dismutase (17.36 ± 0.49 U/mg), catalase (1.42 ± 0.05 U/mg) glutathione (37.56 ± 1.89 μ mol/mg) and reduction in malondialdehyde (7.83 ± 0.21 n mol/mg) level was observed in group receiving 800mg/kg of mep.a as compared to amnesic group. Pimpinella anisum thus improved memory in mice by preventing oxidative stress in brain.
 

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Pakistan Journal of Zoology

August

Vol. 52, Iss. 4, Pages 1225-1630

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