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Melatonin Supplementation in Extender Enhances the Post Thaw Quality of Buffalo Bull Spermatozoa

Melatonin Supplementation in Extender Enhances the Post Thaw Quality of Buffalo Bull Spermatozoa

Asma-ul-Husna1, Muhammad Sajjad Ansari2, Bushra Allah Rakha3, Rabea Ejaz1, Nemat Ullah1 and Shamim Akhter1*

 

1Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi-46300, Pakistan
2Department of Zoology, University of Sargodha, Lyallpur Campus, Faisalabad-38000, Pakistan
3Department of Wildlife Management, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi-46300, Pakistan

*           Corresponding author: sashraf1993@gmail.com 

 

 

ABSTRACT

Oxidative damage to sperm during cryopreservation is one of the main causes of decline in fertilizing potential. Present study aimed at evaluating the role of melatonin in extender for its cryoprotective effect on post-thaw quality of buffalo bull semen. For this purpose, two consecutive ejaculates were collected from three Nili-Ravi buffalo bulls using artificial vagina at weekly intervals for a period of three weeks (three replicates). Qualifying semen ejaculates were diluted (50×106 motile spermatozoa ml-1) in tris citric acid extender with melatonin at 0 (control), 0.1, 0.5, 1, and 1.5 mM. Diluted semen was cooled to 4°C for 2 h and equilibrated for 4 h at 4°C. Straws were then kept over liquid nitrogen vapours for 10 min and plunged in liquid nitrogen for storage. Thawing was performed after 24 h of storage, at 37°C for 30 seconds and post-thaw sperm quality parameters were assessed. Sperm progressive motility, plasma membrane integrity, acrosomal integrity, viability and chromatin integrity was higher (P<0.05) in extender containing melatonin 0.1 mM and 0.5 mM compared to control. In conclusion, the addition of melatonin 0.1-0.5 mM to extender improved the post-thaw quality of buffalo bull semen.

 

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Pakistan Journal of Zoology

December

Vol. 51, Iss. 6, Pages 1999-2399

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