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Kinetic and Parametric Optimization for the Enhanced Production of a Novel Fungal Exo-inulinase under Liquid Culture

Kinetic and Parametric Optimization for the Enhanced Production of a Novel Fungal Exo-inulinase under Liquid Culture

Sikander Ali* and S. Wajiha Khalid

Institute of Industrial Biotechnology, GC University, Lahore

* Corresponding author: dr.sikanderali@gcu.edu.pk

Fig. 1.

Comparison of protein content, sugar concentration and dry cell mass by different filamentous fungal cultures. Temperature 30°C, pH 5.5, time of incubation 72 h, agitation 160 rpm, inoculum size 2 %. Each value is a mean average of three parallel replicates. Bars specify ± standard deviation.

Fig. 2.

Comparison of exo-inulinase activity, protein content, sugar conc. and DCM over different time of incubation by ROMII and PENLI. Temperature 30°C, pH 5.5, agitation 160 rpm, inoculum size 2 %. Each value is a mean average of three parallel replicates. Bars specify ± standard deviation.

Fig. 3.

Effect of various pH on exo-inulinase production from R. oligosporus (ROMII). Temperature 30°C, time of incubation 72 h, agitation 160 rpm, inoculum size 2%. Each value is a mean average of three parallel replicates. Bars specify ± standard deviation.

Fig. 4.

Effect of various agitation rates on exo-inulinase production from R. oligosporus (ROMII). Temperature 30°C, pH 5.5, time of incubation 72 h, inoculum size 2 %. Each value is a mean average of three parallel replicates. Bars specify ± standard deviation.

Fig. 5.

Role of inoculum (age and size) on exo-inulinase production by R. oligosporus (ROMII). Temperature 30°C, pH 5.5, time of incubation 72 h, agitation 120 rpm. Each value is a mean average of three parallel replicates. Bars specify ± standard deviation.

Fig. 6.

The molecular weight of inulinase from R. oligosporus (ROMII) through SDS. Lane 1: Protein ladder, Lane 2: Crude inulinase extract from ROMII with molecular weight 86 kDa and Lane 3: Partially purified inulinase from ROMII with molecular weight 86 kDa.

Pakistan Journal of Zoology

October

Vol. 52, Iss. 5, Pages 1631-2026

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