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Joint Action of Trichoderma hamatum and Difenoconazole on Growth of a Phytopathogen Sclerotinia sclerotiorum under Laboratory Conditions

Joint Action of Trichoderma hamatum and Difenoconazole on Growth of a Phytopathogen Sclerotinia sclerotiorum under Laboratory Conditions

Dan Yü, Chuchu Li, Yü Huang and Zhen Huang*

College of Agriculture, South China Agricultural University, Guangzhou 510642, P.R. China

*      Corresponding author: hzscau@scau.edu.cn

 

ABSTRACT

Sclerotinia sclerotiorum is a serious phytopathogen resulting in significant loss of crop yield. Trichderma hamatum has the capacity to inhibit S. sclerotiorum growth by production of toxic metabolites and mycoparasitism. In vitro bioassays were used to evaluate the efficacy of T. hamatum and difenoconazole alone or its combinations against S. sclerotiorum. The results showed that T. hamatum could infect and destroy the mycelia and sclerotia of S. sclerotiorum by mycoparasitism. The cell-free culture supernatant and ethylacetate extracts from T. hamatum inhibited mycelial growth of S. sclerotiorum, with EC50 value of 0.54 µl/cm2 and 2.99 µg/ml, respectively. The sclerotia production of S. sclerotiorum decreased when treated with ethylacetate extracts. The level of additive inhibition on S. sclerotiorum using both fungal ethylacetate extracts and difenoconazole was directly related to the concentration of each component used in mixtures. Additive effects were observed in all treatments according to the mycelial growth inhibition, Me and Chi-square values. The use of T. hamatum offers a promising and effective co-formulation product or alternative to chemical fungicides in controlling popcorn disease of mulberry.
 

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Pakistan Journal of Zoology

December

Vol. 50, Iss. 6, Pages 1999-2398

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