Isolation of Field Isolate of Equine herpes virus-1 Based on Molecular Detection and Characterization of Glycoprotein D
Isolation of Field Isolate of Equine herpes virus-1 Based on Molecular Detection and Characterization of Glycoprotein D
Abd El-Hamid, M.I1; Seham, A. ElZeedy1; El-Sanousi A.A2, Reda, I.M2, Nehal, S. Saleh1., Abbas, A.M1
ABSTRACT
Equine herpesvirus (EHV)-1 is a pathogen of horses, well known for its ability to induce abortion and nervous system disorders neonatal foal pneumonitis, highly contagious among susceptible horses, with viral Transmission to cohort animals occurring by inhalation of aerosols of virus-laden respiratory secretions. Extensive use of vaccines has not eliminated EHV infections, and the worldwide annual financial burden from these equine pathogens is immense. Clinical signs may occur despite the presence of a virus-specific immune response in the horse. The current study focus on outbreak recorded in El Zahraa Stud for Arabian horses during 2014 and recorded officially for the first time by OIE in Egypt by the notification presented by Chairman, General Organization for Veterinary Services (GOVS), Ministry of Agriculture and Land Reclamation, the samples collected from the stud were either aborted fetal tissues or nasal swaps from apparently healthy animals and previously aborted mares the current study focuses on isolation and molecular characterization of EHV-1 of the local isolate Egypt/VSVRI/Zahraa/2014 using the glycoprotein D of EVH-1 due to its role in virus infectivity and its function in entry of virus into cells and is considered as one of the most potent inducers of virus-neutralizing antibody among the spectrum of EHV-1 proteins based on sequence analysis and multiple alignment revealed single nucleotide substitution at the base pair number 121 from the start codon which give lead to single Amino Acid Substitution from CAG (Glutamine(Gln/Q)) which is considered as a polar Amino Acid to AAG (Lysine(Lys/K)) which is a basic Amino Acid which is very characteristic for the local isolate than the rest of isolate and strains.
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