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Isolation and Molecular Characterization of Medically Important Yeasts Isolated from Poultry Slaughterhouses and Workers

Isolation and Molecular Characterization of Medically Important Yeasts Isolated from Poultry Slaughterhouses and Workers

Eman Mahmoud El-Diasty1,Madeha Abd El-Halim Ibrahimand Ghada Kamal El Khalafawy2,*

1Department of Mycology, Animal Health Research Institute, Dokki, Giza
2Health Radiation Research Department, National Centre for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt

ABSTRACT

Early identification of yeast to the genus and species level is necessary for effective antifungal therapy, and can also facilitate control of infections. A total of 50 isolates were isolated from 60 swab samples collected from two poultry processing plants represented broiler carcasses swabs and worker (hand swabs and nail scrapings). Phenotype-based methods for identifying yeast especially Candida species are often difficult, time-consuming and not enough to identify most yeast species. . Molecular biological techniques provide a useful alternative approach. Concerning with genotypic identification, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was applied for identification of Candida species, Cryptococcus albidus, Saccharomyces, Trichosporn, Torulopsis and Rhodotorula. Using universal primers ITS1-5.8S-ITS4 amplified ITS region. And it yielded a unique PCR size of approximately 376-930 bp. PCR amplicons were digested with enzyme MspI and the generated bans corresponded to the predicted size.
 

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Pakistan Journal of Zoology (Associated Journals)

December

Vol. 49, Iss. 5, Pages 1937-2341

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