A 9-week feeding trial was conducted to investigate the effects of Isaria cicadae Miquel (I. cicadae) on growth performance and meat quality of giant freshwater prawn (Macrobrachium rosenbergii). The prawns were fed with five isonitrogenous (40.54% crude protein) and isolipid (6.16% crude lipid) diets with different I. cicadae levels (0, 100, 200, 400, and 800 mg Kg-1). Results revealed that I. cicadae significantly increased specific growth ratio (SGR) and protein efficiency ratio (PER), while decreased feed intake (FI) and feed conversion ratio (FCR). Meanwhile, I. cicadae significantly increased plasma ALT activity, urea nitrogen, total protein, albumin and globulin contents, while significantly decreased AST activity in 400 mg Kg-1 group. Haemolymph SOD and GSH-PX activity was enhanced significantly in 400 and 800 mg Kg-1 group, respectively, while MDA content was significantly decrease in these two groups. I. cicadae did not affect the muscle cooking loss, but significantly increased the compression loss. Moreover, I. cicadae significantly increased the muscle texture profile including gumminess, hardness and chewiness, and decreased the muscle sensory evaluations including whiteness and succulency. Therefore, 100 mg Kg-1 I. cicadae could improve growth performance and meat quality of giant freshwater prawn, reduce the damage of oxidative stress.