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Immunolocalization and Expression of Androgen Receptor in the Oviduct of Laying Hens

Immunolocalization and Expression of Androgen Receptor in the Oviduct of Laying Hens

Bian Xunguang1,2, Li Jiancheng1, Xu Chu1 and Yang Li2,3,4,*

1School of Ocean and Biological Engineering, Yancheng Teachers University, Yancheng, Jiangsu Province, People’s Republic of China
2Jiangsu Key Laboratory for Bioresources of Saline Soils, Yancheng Teachers University, Yancheng 224051, Jiangsu Province, People’s Republic of China
3Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Yancheng Teachers University, Yancheng, Jiangsu Province, People’s Republic of China
4Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers University, Yancheng, Jiangsu Province, People’s Republic of China

*      Corresponding author: bxguang8311@126.com

ABSTRACT

Androgen plays an important role in animals, and its role is mainly via androgen receptor (AR) to achieve. In this paper, the expression of AR mRNA in various parts of the oviduct was analyzed by qPCR. Oviductal structure was observed by H&E staining. The distribution of AR in the cells was detected by immunohistochemistry. The results showed that the expression of AR mRNA was found in the infundibulum, magnum, isthmus, uterus and vagina of the oviduct. The expression of the AR mRNA in infundibulum was the strongest, and the vaginal expression was weak. The immunohistochemical results was consistent with the molecular expression of AR mRNA, the positive reaction in the infundibulum, isthmus and uterus is more intense, followed by the magnum, the vaginal reaction is weak. Positive responses in the sections were mainly focused on luminal epithelium, glands and vascular epithelial cells. This study revealed the molecular expression of AR and the distribution of intracellular proteins in the oviduct of the hen, which provides an important basis for the regulation of the physiological activities of poultry oviduct and the molecular mechanism of sperm storage.
 

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Pakistan Journal of Zoology

December

Vol. 49, Iss. 6, Pages 1937-2341

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